Genome Editing in the Cricket, Gryllus bimaculatus
Genome Editing in the Cricket, Gryllus bimaculatus
复制标题
DOI：
10.1007/978-1-4939-7128-2_18
复制
发表时间：
2017-01-01
期刊：
影响因子：
--
通讯作者：
Mito, Taro
中科院分区：
文献类型：
--
作者：
Watanabe, Takahito;Noji, Sumihare;Mito, Taro
半变态或不完全变态的昆虫是昆虫发生学的基础，包括许多有益和有害的种类。蟋蟀是半变态昆虫的一个新模式，基于RNA干扰（RNAi）的基因功能分析和转基因技术的成功。利用该物种的基因组编辑技术将极大地促进功能基因组学研究。基因组编辑已被证明是在各种物种中进行位点特异性基因组操作的有效方法。在这里，我们描述了一个基因组编辑的协议，包括基因敲除和基因敲入在G。用于功能基因组学研究。
Hemimetabolous, or incompletely metamorphosing, insects are phylogenetically basal and include many beneficial and deleterious species. The cricket, Gryllus bimaculatus, is an emerging model for hemimetabolous insects, based on the success of RNA interference (RNAi)-based gene-functional analyses and transgenic technology. Taking advantage of genome editing technologies in this species would greatly promote functional genomics studies. Genome editing has proven to be an effective method for site-specific genome manipulation in various species. Here, we describe a protocol for genome editing including gene knockout and gene knockin in G. bimaculatus for functional genomics studies.