Molecular Characterization of Campylobacter jejunifrom Patients with Guillain-Barré and Miller Fisher Syndromes
Molecular Characterization of Campylobacter jejunifrom Patients with Guillain-Barré and Miller Fisher Syndromes
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吉兰-巴利综合征和米勒·费希尔综合征患者空肠弯曲菌的分子特征
DOI：
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发表时间：
2000
影响因子：
9.4
通讯作者：
A. van Belkum
中科院分区：
文献类型：
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作者：
H. Endtz;C. Ang;N. van den Braak;B. Duim;Alan Rigter;Lawrence J. Price;David L. Woodward;Frank G. Rodgers;Wendy M. Johnson;Jaap A. Wagenaar;Bart C. Jacobs;H. Verbrugh;A. van Belkum
空肠弯曲杆菌已被确定为格林-巴氏综合征（GBS）和米勒-费舍尔综合征（MFS）先前感染的主要原因。感染特定类型空肠梭菌后，发生GBS或MFS的风险可能更高。采用Penner和Lior血清分型、flaA基因PCR产物限制性内切片段长度多态性分析、脉冲场凝胶电泳和随机扩增多态性DNA分析等方法，对来自荷兰和比利时的18株GBS或mfs相关空肠C. jejuni菌株和17株对照菌株进行克隆性分析。血清分型显示10种不同的O型和7种不同的Lior型，表明缺乏血清型聚类。发现了两种新的O血清型，O:35和O:13/65，以前与GBS或MFS无关。18株毒株中2株检测到O:19血清型，0株检测到O:41血清型。所有基因型方法的结果也显示出很大的异质性。本研究未发现GBS或MFS相关菌株聚集，也未发现能够将致病性GBS或MFS与非GBS或非MFS相关肠炎菌株分离的分子标记。含唾液酸脂多糖（LPS）被认为通过与人周围神经神经节苷脂的分子模拟参与了GBS或MFS的触发。因此，进一步表征GBS或mfs相关的空肠梭菌应该针对参与脂多糖合成和唾液酸掺入的基因。
ABSTRACT Campylobacter jejuni has been identified as the predominant cause of antecedent infection in Guillain-Barrésyndrome (GBS) and Miller Fisher syndrome (MFS). The risk of developing GBS or MFS may be higher after infection with specific C. jejuni types. To investigate the putative clonality, 18 GBS- or MFS-related C. jejuni strains from The Netherlands and Belgium and 17 control strains were analyzed by serotyping (Penner and Lior), restriction fragment length polymorphism analysis of PCR products of the flaA gene, amplified fragment length polymorphism analysis, pulsed-field gel electrophoresis, and randomly amplified polymorphic DNA analysis. Serotyping revealed 10 different O serotypes and 7 different Lior serotypes, thereby indicating a lack of serotype clustering. Two new O serotypes, O:35 and O:13/65, not previously associated with GBS or MFS were found. Serotype O:19 was encountered in 2 of 18 strains, and none was of serotype O:41. The results of all genotypic methods also demonstrated substantial heterogeneity. No clustering of GBS- or MFS-related strains occurred and no molecular marker capable of separating pathogenic GBS or MFS from non-GBS- or non-MFS-related enteritis strains could be identified in this study. Sialic-acid-containing lipopolysaccharides (LPS) are thought to be involved in the triggering of GBS or MFS through molecular mimicry with gangliosides in human peripheral nerves. Therefore, further characterization of GBS- or MFS-related C. jejuni should target the genes involved in the synthesis of LPS and the incorporation of sialic acid.
影响因子：
14.5
作者：
HO, TW;MISHU, B;MCKHANN, GM
通讯作者：
MCKHANN, GM