Role of binding energy with coenzyme A in catalysis by 3-oxoacid coenzyme A transferase.
Role of binding energy with coenzyme A in catalysis by 3-oxoacid coenzyme A transferase.
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与辅酶 A 的结合能在 3-含氧酸辅酶 A 转移酶催化中的作用。
DOI：
10.1021/bi00037a005
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发表时间：
1995
期刊：
影响因子：
2.9
通讯作者：
Jencks,WP
中科院分区：
文献类型：
--
作者：
Whitty,A;Fierke,CA;Jencks,WP
材料和方法材料。从辅酶A转移酶或E，琥珀酰辅酶A：3-氧酸辅酶A转移酶(EC 2.8)得到琥珀酸、醋酸钾和乙二胺四乙酸二钠(EDTA)。3‘，5’-二硫代二(2-硝基苯甲酸)；3‘，5’-ADP，腺苷3‘，5’-二磷酸二钠；5‘-ADP，腺苷5’-一磷酸二钠；acac-CoA，乙酰乙酰-CoA；Succ-CoA，琥珀酰-CoA；acac，乙酰乙酸；acac-，乙酰乙酰；Succ，琥珀酸；Succ-，琥珀酰基；NAC，A‘-乙酰半胱胺；NaA，N-乙酰半胱胺；金属硫醇，N-乙酰丙酸甲酯；Pant，Pantetheine；RSH，任何这些硫醇；E-CoA、E-NAC、E-NAA、E-MMP、E-PANT和E-SR，分别含有活性部位共价结合的CoA、N-乙酰半胱胺、VV-乙酰丙氨酸、硫代丙酸甲酯、泛茶氨酸或上述任何硫醇的硫醇酶。
MATERIALS AND METHODSMaterials. Succinic acid, potassium acetate and disodium ethylenediaminetetraacetate (EDTA) were obtained from1 Abbreviations: CoA transferase or E, succinyl-CoA: 3-oxoacid coenzyme A transferase (EC 2.8. 3.5); DTNB, 5, 5'-dithiobis (2-nitrobenzoic acid); 3', 5'-ADP, disodium adenosine 3', 5'-diphosphate; 5'-ADP, disodium adenosine 5'-monophosphate; AcAc-CoA, acetoacetyl-CoA; Succ-CoA, succinyl-CoA; AcAc, acetoacetic acid; AcAc-, acetoacetyl; Succ, succinic acid; Succ-, succinyl; NAC, A'-acetylcysteamine; NAA, N-acetylaletheine; MMP, methyl mercaptopropionate; Pant, pantetheine; RSH, any of these thiols; E-CoA, E-NAC, E-NAA, E-MMP, E-Pant, and E-SR, enzyme thiol esters containing, respectively, CoA, N-acetylcysteamine, vV-acetylaletheine, methyl mercaptopropionate, pan-tetheine, or any of the above thiols covalently bound in the active site.