Plasma lipid peroxidation in sporadic Parkinson's disease.: Role of the L-dopa
Plasma lipid peroxidation in sporadic Parkinson's disease.: Role of the L-dopa
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DOI：
10.1016/j.jns.2005.08.016
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发表时间：
2006-01-15
影响因子：
4.4
通讯作者：
Vives, F
中科院分区：
文献类型：
--
作者：
Agil, A;Durán, R;Vives, F
氧化应激在帕金森病（PD）等神经退行性疾病的发病机制中发挥着重要作用。有多种测量氧化应激的方法，其中脂质过氧化 (LPO) 是最常用的方法之一。通过荧光分光光度法测定了 52 名散发性 PD 患者和 40 名对照者的内源性血浆 LPO。为了了解脂质过氧化的最大能力，还测量了与 Fe2+/H2O2（外源 LPO）共孵育后的 LPO。所有 PD 患者均服用左旋多巴，并额外研究了这种治疗对 LPO 水平的影响。还分析了尿液儿茶酚胺及其主要代谢物，并统计研究了它们与 LPO 可能的相关性。 PD组内源性血浆LPO水平比对照组高33%（P < 0.001）。与对照组相比，PD 患者的外源血浆或氧化能力也较高（20%，P < 0.05）。左旋多巴的摄入量与内源性和外源性血浆LPO呈负剂量相关。总之，PD患者血浆中LPO水平升高，并且比对照组更容易发生过氧化反应。结果还表明左旋多巴具有抗氧化作用。 (c) 2005 Elsevier B.V. 保留所有权利。
Oxidative stress plays an important role in the pathogenesis of neurodegenerative diseases, such as Parkinson's disease (PD). There are several methods to measure oxidative stress, being lipid peroxidation (LPO) one of the most frequently used. Endogenous Plasma LPO was determined by a spectrofluorimetric method in fifty two patients with sporadic PD and in forty controls. To know the maximum capacity of lipids to peroxidate, LPO was also measured after co-incubation with Fe2+/H2O2 (exogenous LPO). All PD patients were taken L-dopa and the effect of this treatment on LPO levels was additionally studied. Urine catecholamines and their main metabolites were also analyzed, and their possible correlation to LPO statistically studied. Endogenous plasma LPO levels were 33% higher in PD group than in control group (P < 0.001). Exogenous plasma or oxidizability was also higher in PD patients compared to controls (20%, P < 0.05). The intake of L-dopa was negatively dose-related to endogenous and exogenous plasma LPO. In conclusion, plasma of PD patients has elevated levels of LPO and also is more prone to peroxidation than that in the control group. The results also suggest an antioxidant effect of L-dopa. (c) 2005 Elsevier B.V. All rights reserved.