Novel, rapid, and inexpensive cell-based quantification of antimalarial drug efficacy
Novel, rapid, and inexpensive cell-based quantification of antimalarial drug efficacy
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DOI：
10.1128/aac.48.5.1807-1810.2004
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发表时间：
2004-05-01
影响因子：
4.9
通讯作者：
Roepe, PD
中科院分区：
文献类型：
--
作者：
Bennett, TN;Paguio, M;Roepe, PD
我们报告了一种新的SYBR绿色I平板法的发展，用于分析抗疟药物对红细胞内恶性疟原虫的活性。该测定比常规放射性示踪剂（例如，[H-3]次黄嘌呤和[H-3]乙醇胺）的测定或基于恶性疟原虫乳酸脱氢酶活性的测定。该试验显著提高了抗疟药物发现工作的进展速度。
We report on the development of a new SYBR Green I-based plate assay for analyzing the activities of antimalarial drugs against intraerythrocytic Plasmodium falciparum. This assay is considerably faster, less labor-intensive, and less expensive than conventional radiotracer (e.g., [H-3]hypoxanthine and [H-3]ethanolamine)-based assays or P. falciparum lactate dehydrogenase activity-based assays. The assay significantly improves the pace at which antimalarial drug discovery efforts may proceed.