Human tissue factor: cDNA sequence and chromosome localization of the gene.
Human tissue factor: cDNA sequence and chromosome localization of the gene.
复制标题
人体组织因子：基因的 cDNA 序列和染色体定位。
DOI：
10.1021/bi00391a004
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发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
Sadler,JE
中科院分区：
文献类型：
--
作者：
Scarpati,EM;Wen,D;BrozeJr,GJ;Miletich,JP;Flandermeyer,RR;Siegel,NR;Sadler,JE
华盛顿大学医学院霍华德·休斯医学研究所和医学与生物化学系，圣路易斯，密苏里州63110；加州大学旧金山，加利福尼亚94143，产科，妇科和生殖科学系；孟山都公司，圣路易斯，密苏里州63198摘要：利用兔多克隆抗人组织因子免疫球蛋白g对人胎盘xhtf1 cDNA文库进行了筛选，以表达组织因子抗原。在筛选的400万个重组克隆中，有一个阳性克隆XHTF8表达的蛋白与真实的人脑组织因子具有相同的表位。XHTF8的1.1千碱基cDNA插入片段编码了一个包含人脑组织因子氨基末端蛋白序列的肽段。Northern blotting鉴定出胎盘poly (a)+ RNA的主要mRNA种为2.2千碱基，次要mRNA种为~ 3.2千碱基。在人脑和人单核细胞U937细胞系中仅检测到2.2千碱基mRNA。在U937细胞中，暴露于12-肉豆肉酸13-乙酸磷后，组织因子mRNA的数量增加了数倍。与XHTF8的cDNA插入片段杂交，选择更多cDNA克隆。这些重叠的分离株跨越组织因子cDNA序列的2177个碱基对，包括75个碱基对的5‘非编码区，885个碱基对的开放阅读框，一个停止密码子，1141个碱基对的3’非编码区和一个聚(a)尾。开放阅读框编码一个由295个氨基酸组成的33千道尔顿蛋白质。预测的序列包括32或34个氨基酸的信号肽，217或219个氨基酸的可能的细胞外因子VII结合域，23个氨基酸的跨膜段和21个氨基酸的细胞质尾部。有三个潜在的糖基化位点，序列为Asn-X-Thr/Ser。3'-非编码区包含一个反向Alu家族重复序列。组织因子基因定位于染色体
Howard Hughes Medical Institute and Departments of Medicine and Biological Chemistry, Washington University School of Medicine, St. Louis, Missouri 63110, Department of Obstetrics, Gynecology, and Reproductive Science, University of California, San Francisco, California 94143, and Monsanto Company, St. Louis, Missouri 63198 Received June 3, 1987; Revised Manuscript Received June 25, 1987 abstract: A human placenta cDNA library in Xgtl 1 was screened for the expression of tissue factor antigens with rabbit polyclonal anti-human tissue factor immunoglobulin G. Among 4 million recombinant clones screened, one positive, XHTF8, expressed a protein that shared epitopes with authentic human brain tissue factor. The 1.1-kilobase cDNA insert of XHTF8 encoded a peptide that containedthe amino-terminal protein sequence of human brain tissue factor. Northern blotting identified a major mRNA species of 2.2 kilobases and a minor species of~ 3.2 kilobases in poly (A)+ RNA of placenta. Only 2.2-kilobase mRNA was detected in human brain and in the human monocytic U937 cell line. In U937 cells, the quantity of tissue factor mRNA was increased severalfold by exposure of the cells to phorbol 12-myristate 13-acetate. Additional cDNA clones were selected by hybridizationwith the cDNA insert of XHTF8. These overlapping isolates span 2177 base pairs of the tissue factor cDNA sequence that includes a 5'-noncoding region of 75 base pairs, an open reading frame of 885 base pairs, a stop codon, a 3'-noncoding region of 1141 base pairs, and a poly (A) tail. The open reading frame encodes a 33-kilodalton protein of 295 amino acids. The predicted sequence includes a signal peptide of 32 or 34 amino acids, a probable extracellular factor VII binding domain of 217 or 219 amino acids, a transmembrane segment of 23 amino acids, and a cytoplasmic tail of 21 amino acids. There are three potential glycosylation sites with the sequence Asn-X-Thr/Ser. The 3'-noncoding region contains an inverted Alu family repetitive sequence. The tissue factor gene was localized to chromosome