Endothelin-1 induces prostacyclin release from bovine aortic endothelial cells.
Endothelin-1 induces prostacyclin release from bovine aortic endothelial cells.
复制标题
Endothelin-1 诱导牛主动脉内皮细胞释放前列环素。
DOI：
10.1016/0006-291x(91)91964-e
复制
发表时间：
1991
影响因子：
3.1
通讯作者：
P. Sirois
中科院分区：
文献类型：
--
作者：
J. Filep;B. Battistini;Y. P. Côté;A. Beaudoin;P. Sirois
本文研究了内皮素-1（ET-1）对培养的牛主动脉内皮细胞释放前列环素的影响。ET-1诱导前列环素的稳定代谢产物6-ketoPGF 1 α释放呈时间和剂量依赖性，EC 50值为3.0 ± 0.9 nM（n=6）。高达500 nM浓度的ET-1不影响细胞完整性。用10 μM吲哚美辛预孵育细胞30 min可抑制ET-1（100 nM）诱导的前列环素释放90%。这些结果表明，ET-1可能通过受体介导的机制直接刺激内皮细胞释放前列环素。
The effects of endothelin-1 (ET-1) on the release of prostacyclin from cultured bovine aortic endothelial cells were studied. ET-1 induced a time- and dose-dependent release of 6-keto PGF1α, the stable metabolite of prostacyclin, with an apparent EC50value of 3.0 ± 0.9 nM (n=6). ET-1 up to a concentration of 500 nM did not affect cellular integrity. Preincubation of the cells for 30 min with 10 μM indomethacin inhibited ET-1 (100 nM) - induced prostacyclin release by 90 %. These findings indicate that ET-1 can directly stimulate prostacyclin release from endothelial cells probably through a receptor mediated mechanism.
DOI：
10.1172/jci114505
发表时间：
1990-03
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
M. Simonson;Michael J. Dunn
通讯作者：
M. Simonson;Michael J. Dunn