In Vitro Biocompatibility of Endodontic Sealers Incorporating Antibacterial Nanoparticles
In Vitro Biocompatibility of Endodontic Sealers Incorporating Antibacterial Nanoparticles
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DOI：
10.1155/2012/858073
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发表时间：
2012-01-01
影响因子：
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通讯作者：
Houri-Haddad, Yael
中科院分区：
文献类型：
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作者：
Abramovitz, Itzhak;Beyth, Nurit;Houri-Haddad, Yael
牙髓病的主要病因是细菌。目前，消毒是通过在充填之前清洁根管系统来实现的。根管治疗后，根管充填物没有任何抗菌作用。根管治疗后，根管治疗的效果会更好。为此目的，可以使用季铵化聚乙烯亚胺纳米颗粒，其是抗菌聚合物、生物相容性、非挥发性和稳定的。本研究的目的是检查添加QPEI对AH Plus、Epiphany和GuttaFlow牙髓封闭剂细胞毒性的影响。检查了这些密封剂对RAW 264.7巨噬细胞和L-929成纤维细胞系增殖以及对巨噬细胞产生TNF α的影响。使用比色XTT测定评价细胞活力。通过双位点ELISA测定细胞因子的存在。结果表明，1%浓度的QPEI不会损害巨噬细胞和成纤维细胞系中检查的密封剂的基本性质。将1% QPEI掺入密封剂中不会损害其生物相容性。QPEI是一种潜在的临床候选药物，可改善密封剂的抗菌活性，而不会增加细胞毒性。
The main cause of endodontic disease is bacteria. Disinfection is presently achieved by cleaning the root canal system prior to obturation. Following setting, root canal filling is devoid of any antibacterial effect. Endodontic sealers with antimicrobial properties yet biocompatible may enhance root canal therapy. For this purpose, quaternized polyethylenimine nanoparticles which are antibacterial polymers, biocompatible, nonvolatile, and stable may be used. The aim of the present study was to examine the impact of added QPEI on the cytotoxicity of AH Plus, Epiphany, and GuttaFlow endodontic sealers. The effect of these sealers on the proliferation of RAW 264.7 macrophage and L-929 fibroblast cell lines and on the production of TNF alpha from macrophages was examined. Cell vitality was evaluated using a colorimetric XTT assay. The presence of cytokines was determined by two-site ELISA. Results show that QPEI at 1% concentration does not impair the basic properties of the examined sealers in both macrophages and fibroblast cell lines. Incorporation of 1% QPEI into the sealers did not impair their biocompatibility. QPEI is a potential clinical candidate to improve antibacterial activity of sealers without increasing cytotoxicity.