High-performance liquid chromatographic determination of phenolic compounds in rice.
High-performance liquid chromatographic determination of phenolic compounds in rice.
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DOI：
10.1016/j.chroma.2004.11.075
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发表时间：
2005-01
期刊：
影响因子：
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通讯作者：
Su Tian;K. Nakamura;T. Cui;H. Kayahara
中科院分区：
文献类型：
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作者：
Su Tian;K. Nakamura;T. Cui;H. Kayahara
建立了测定大米中6′-O-阿魏酰蔗糖、6′-O-芥子酰蔗糖、阿魏酸、芥子酸、对香豆酸、绿原酸、咖啡酸、原儿茶酸、羟基苯甲酸、香草酸和胡萝卜酸的方法。大米样品用70%乙醇提取，过滤，脱脂。使用C18硅胶柱对脱脂水溶液进行固相萃取;在该过程中没有分析物损失。通过HPLC和HPLC-ESI-MS直接分析70%酸性甲醇洗脱液。使用C18反相色谱柱，采用0.025%三氟乙酸纯化水（A）-乙腈（B）梯度洗脱，分离酚类化合物。移动的相为0 min，B; 5 min，B; 15 min，B; 22 min，B; 38 min，B，流速为0.8ml/min，检测限为0.10 ~ 0.35ng/次（5μl）。同时测定这些酚类化合物的相对标准偏差为0.22-3.95%，回收率为99-108%。将该方法应用于糙米和在32°C水中浸泡不同时间的发芽糙米中酚类化合物的分析。
A method has been developed for the determination of 6′-O-feruloylsucrose, 6′-O-sinapoylsucrose, ferulic acid, sinapinic acid, p-coumaric acid, chlorogenic (3-caffeoylquinic) acid, caffeic acid, protocatechuic acid, hydroxybenzoic acid, vanillic acid, and syringic acid in rice. The rice samples were extracted with 70% ethanol, filtered, and defatted. The defatted aqueous solution was subjected to solid-phase extraction using a C18silica gel cartridge; no analyte was lost in this procedure. The 70% acidic methanol elution was analyzed directly by HPLC and HPLC-ESI-MS. Phenolic compounds were separated with a C18reversed-phase column by gradient elution using 0.025% trifluoroacetic acid in purified water (A)—acetonitrile (B) (0min, 5% B; 5min, 9% B; 15min, 9% B; 22min, 11% B; and 38min, 18% B) as the mobile phase at a flow rate of 0.8ml/min. Detection limits ranged from 0.10 to 0.35ng per injection (5μl). Relative standard deviations of 0.22–3.95% and recoveries of 99–108% were obtained for simultaneous determination of these phenolic compounds. This method was applied to analysis of phenolic compounds in brown rice and germinated brown rice soaked in 32°C water for varying durations.