The Circadian Clock Gene Circuit Controls Protein and Phosphoprotein Rhythms in Arabidopsis thaliana.
The Circadian Clock Gene Circuit Controls Protein and Phosphoprotein Rhythms in Arabidopsis thaliana.
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DOI：
10.1016/j.mcpro.2021.100172
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发表时间：
2022-01
期刊：
影响因子：
--
通讯作者：
Millar AJ
中科院分区：
文献类型：
--
作者：
Krahmer J;Hindle M;Perby LK;Mogensen HK;Nielsen TH;Halliday KJ;van Ooijen G;Le Bihan T;Millar AJ
24小时的昼夜节律控制着许多真核生物的mRNA水平，而它们更稳定的蛋白质水平并不能反映RNA的节律，强调了测试蛋白质丰度和修饰的昼夜节律的必要性。在这里，我们提供了恒光条件下拟南芥植物的昼夜节律蛋白质组和磷蛋白质组时间序列，估计只有0.4%的量化蛋白质是有节律性的，但量化的磷酸位点的比例要大得多。大约一半的节律性磷酸化位点在主观黎明时最容易被磷酸化，我们称之为“磷酸化黎明”。SnRK/CDPK蛋白激酶家族的成员是候选的调节者。CCA1过度表达使时钟基因回路失效的品系缺乏大部分昼夜蛋白磷酸化。然而，尽管CCA1过表达，少数几个磷酸化位点仍然倾向于在接近主观黎明时达到丰度峰值，这表明典型时钟机制对于大多数但可能不是所有蛋白质磷酸化节律是必要的。为了测试我们的数据集的潜在功能相关性，我们以双功能酶果糖-6-磷酸-2-激酶/磷酸酶(F2KP)为例进行了仿磷实验。不同蛋白质靶标的有节奏的磷酸化是由时钟基因电路控制的，这意味着在植物中传递昼夜节律信息的翻译后机制。有或没有功能时钟的植物的昼夜(磷)蛋白质组学时间进程。大多数蛋白质丰度/磷酸化节律都需要转录振荡器。大多数节律性亚磷酸盐在主观黎明(“磷黎明”)前后达到峰值。代谢酶F2KP的磷酸化丝氨酸具有功能相关性。植物有昼夜节律，由转录因子网络驱动。因此，生物钟的研究主要集中在转录调控上。然而，非转录过程也起到了作用。因此，我们在这里介绍了昼夜节律(磷酸)蛋白质组学的时间进程。我们发现了具有不同生物学作用的节律性磷酸化蛋白质，并展示了一个例子的功能相关性。这些节奏中的大多数都需要转录振荡器。此外，大多数节律性磷酸化在黎明前后达到峰值，这是我们分析的重点。这些结果增加了我们对非转录昼夜节律过程的了解。
Twenty-four-hour, circadian rhythms control many eukaryotic mRNA levels, whereas the levels of their more stable proteins are not expected to reflect the RNA rhythms, emphasizing the need to test the circadian regulation of protein abundance and modification. Here we present circadian proteomic and phosphoproteomic time series from Arabidopsis thaliana plants under constant light conditions, estimating that just 0.4% of quantified proteins but a much larger proportion of quantified phospho-sites were rhythmic. Approximately half of the rhythmic phospho-sites were most phosphorylated at subjective dawn, a pattern we term the “phospho-dawn.” Members of the SnRK/CDPK family of protein kinases are candidate regulators. A CCA1-overexpressing line that disables the clock gene circuit lacked most circadian protein phosphorylation. However, the few phospho-sites that fluctuated despite CCA1-overexpression still tended to peak in abundance close to subjective dawn, suggesting that the canonical clock mechanism is necessary for most but perhaps not all protein phosphorylation rhythms. To test the potential functional relevance of our datasets, we conducted phosphomimetic experiments using the bifunctional enzyme fructose-6-phosphate-2-kinase/phosphatase (F2KP), as an example. The rhythmic phosphorylation of diverse protein targets is controlled by the clock gene circuit, implicating posttranslational mechanisms in the transmission of circadian timing information in plants. Circadian (phospho)proteomics time courses of plants with or without functional clock. Most protein abundance/phosphorylation rhythms require a transcriptional oscillator. The majority of rhythmic phosphosites peak around subjective dawn (“phospho-dawn”). A phosphorylated serine of the metabolic enzyme F2KP has functional relevance. Plants have circadian rhythms, driven by a transcription factor network. Circadian clock research has therefore focused on transcriptional regulation. However, nontranscriptional processes also play a role. Therefore, we here present circadian (phospho)proteomics time courses. We find rhythmically phosphorylated proteins with diverse biological roles and demonstrate functional relevance of one example. Most of these rhythms require the transcriptional oscillator. Moreover, most rhythmic phosphorylations peak around dawn, which is a focus of our analysis. These results increase our knowledge of nontranscriptional circadian processes.
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影响因子：
4.3
作者：
Fogelmark K;Troein C
通讯作者：
Troein C
影响因子：
11.3
作者：
Baker CL;Loros JJ;Dunlap JC
通讯作者：
Dunlap JC
影响因子：
5.6
作者：
Curran A;Chang IF;Chang CL;Garg S;Miguel RM;Barron YD;Li Y;Romanowsky S;Cushman JC;Gribskov M;Harmon AC;Harper JF
通讯作者：
Harper JF
影响因子：
12.3
作者：
Covington, Michael F.;Maloof, Julin N.;Straume, Marty;Kay, Steve A.;Harmer, Stacey L.
通讯作者：
Harmer, Stacey L.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y