"Electrokinetic Analyte Transport Assay" for α-fetoprotein immunoassay integrates mixing, reaction and separation on-chip
"Electrokinetic Analyte Transport Assay" for α-fetoprotein immunoassay integrates mixing, reaction and separation on-chip
复制标题
DOI：
10.1002/elps.200700898
复制
发表时间：
2008-04-01
期刊：
影响因子：
2.9
通讯作者：
Satomura, Shinji
中科院分区：
文献类型：
--
作者：
Kawabata, Tomohisa;Wada, Henry G.;Satomura, Shinji
A rapid and highly sensitive CE immunoassay method integrating mixing, reaction, separation, and detection on-chip is described for the measurement of alpha-fetoprotein (AFP), a liver cancer marker in blood. Antibody-binding reagents, consisting of 245-bp DNA coupled anti-AFP WA1 antibody (DNA-WA1) and HiLyte dye-labeled anti-AFP WA2 antibody (HiLyte-WA2), and AFP-containing sample were filled into adjacent zones of a chip channel defined by the laminar flow lines of the microfluidic device using pressure-driven flow. The channel geometry was thus used to quantitatively aliquot the reagents and sample into the chip. DNA-WA1 was electrokinetically concentrated in the channel and sequentially transported through the AFP-sample zone and HiLyte-WA2 zone by ITP in such a manner that the AFP sandwich immune complex formation took place in the sample and HiLyte-WA2 zones. The sandwich AFP immune complex was then detected by LIF after CGE in a separation channel that was arranged downstream of the reaction channel. AFP was detected within 136 s with a detection sensitivity of 5 pM. The on-chip immunoassay described here, applying ITP concentration, in-channel reaction, and CGE separation, has the potential of providing a rapid and sensitive method for both clinical and research applications.
