Genome and Hormones:: Gender Differences in Physiology -: Selected Contribution:: Estrogen receptor-α antisense decreases brain estrogen receptor levels and affects ventilation in male and female rats
Genome and Hormones:: Gender Differences in Physiology -: Selected Contribution:: Estrogen receptor-α antisense decreases brain estrogen receptor levels and affects ventilation in male and female rats
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DOI：
10.1152/jappl.2001.91.4.1886
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发表时间：
2001-10-01
影响因子：
3.3
通讯作者：
Schlenker, EH
中科院分区：
文献类型：
--
作者：
Inamdar, SR;Eyster, KM;Schlenker, EH
我们假设，雌激素受体（ER）-α mRNA的反义寡脱氧核苷酸（ODN）的管理，降低ER蛋白在新生大鼠脑，改变了性别特异性的天门冬氨酸在大鼠的排泄反应，并抵消睾酮比例（TP）的影响，在女性。一日龄大鼠幼鼠脑室注射载体、反义ER ODN或乱序ODN对照。其他组的雌性动物接受TP或溶剂和三种处理之一。脑ER蛋白水平在反义ODN后6 h下降65%，24 h下降35%。除ER ODN给药组雌性和TP溶剂给药组雌性外，天冬氨酸降低了所有组断奶雄性和雌性动物的通气量。天冬氨酸降低通气在所有组的成年女性，除了那些给予TP和男性。断奶ER ODN治疗的大鼠较短，体重低于对照组。只有成年ER ODN治疗的男性表现出这些特征。因此，新生儿ER影响天冬氨酸调节呼吸和身体生长的性别特异性和发展的方式。
We hypothesized that administration of an antisense oligodeoxynucleotide (ODN) to estrogen receptor (ER)-alpha mRNA decreases the ER protein in the neonatal rat brain, alters the sex-specific ventilatory responses to aspartic acid in rats, and counteracts the effects of testosterone proportionate (TP) in females. One-day-old rat pups were injected intraventricularly with vehicle, antisense ER ODN, or scrambled ODN control. Additional groups of females received TP or vehicle and one of the three treatments. Brain ER protein levels were decreased by 65% at 6 h and 35% at 24 h after antisense ODN. Aspartic acid decreased ventilation in all groups of weanling males and females except ER ODN-treated females and TP-vehicle-treated females. Aspartic acid decreased ventilation in all groups of adult females except those given TP and in males. Weanling ER ODN-treated rats were shorter and weighed less than controls. Only adult ER ODN-treated males exhibited these traits. Thus neonatal ER affects aspartic acid modulation of breathing and body growth in a sex-specific and developmental manner.