Ephedra Herb, Mao, Inhibits Antigen-Induced Mast Cell Degranulation by Induction of the Affinity Receptor for IgE Internalization
Ephedra Herb, Mao, Inhibits Antigen-Induced Mast Cell Degranulation by Induction of the Affinity Receptor for IgE Internalization
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麻黄草 (Mao) 通过诱导 IgE 内化亲和受体来抑制抗原诱导的肥大细胞脱粒
DOI：
10.1007/s11095-021-03020-0
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发表时间：
2021
影响因子：
3.7
通讯作者：
Suzuki Ryo
中科院分区：
文献类型：
--
作者：
Nagata Yuka;Ando Hirokazu;Sasaki Yohei;Suzuki Ryo
目的麻黄具有较强的抗过敏作用。本研究采用体外培养的肥大细胞（MC）和MC依赖性过敏反应的体内模型，探讨毛泽东抗过敏性炎症的机制。方法用抗2，4-二硝基苯酚（DNP）免疫球蛋白E（IgE）预致敏骨髓来源的MC（BMMCs），并用抗原（Ag; DNP-人血清白蛋白）攻击。在有/无Mao处理的情况下评估脱粒反应和细胞表面高亲和力IgE受体（FcεRI）表达。被动全身过敏反应（PSA）处理的小鼠给予毛和病理生理反应进行了evaluated.ResultsMao抑制银诱导的BMMC脱粒，但不多克隆激活佛波酯12-肉豆蔻酸酯13-乙酸酯（PMA）和离子霉素，表明毛抑制IgE依赖的激活BMMC。Mao处理的BMMCs表面IgE及其受体FcεRI的表达显著降低。亚细胞定位分析显示，Mao诱导BMMC中FcεRI内化而不脱颗粒。在PSA小鼠模型中，Mao给药预防了抗原诱导的体温过低。结论Mao可诱导MC内化Fc εRI，从而抑制Ag诱导的IgE依赖性脱颗粒。毛泽东对MC脱颗粒的抑制作用可能为变态反应性疾病的治疗提供一种新的途径。
PurposeEphedra herb (Mao) exerts potent anti-allergic effects. This study aimed to examine the underlying mechanisms of Mao on allergic inflammation usingin vitrocultured mast cells (MCs) and anin vivomodel of MC-dependent anaphylaxis.MethodsBone marrow-derived MCs (BMMCs) were presensitized with anti-2,4-dinitrophenol (DNP) immunoglobulin E (IgE) and challenged with antigens (Ag; DNP-human serum albumin). Degranulation responses and cell surface high-affinity receptor for IgE (FcεRI) expression were assessed with/without Mao treatment. Passive systemic anaphylaxis (PSA)-treated mice were administered Mao and the pathophysiological responses were evaluated.ResultsMao inhibited Ag-induced BMMC degranulation, but not polyclonal activation with phorbol 12-myristate 13-acetate (PMA) and ionomycin, indicating that Mao inhibits IgE-dependent activation of BMMCs. Mao-treated BMMCs exhibited significant reductions in expression of surface IgE and its receptor FcεRI. Analysis of subcellular localization revealed that Mao induces FcεRI internalization in BMMCs without degranulation. In the PSA mouse model, Mao administration prevented antigen-induced hypothermia. Mao administration significantly reduced cell surface expression of IgE-bound FcεRI on peritoneal MCs.ConclusionsMao induced FcεRI internalization in MCs, thereby inhibiting Ag-induced IgE-dependent degranulation. The inhibitory effects of Mao on MC degranulation may offer a novel therapeutic approach for allergic diseases.