Multiple molecular approaches yield no evidence for sex-determining genes in lake sturgeon (Acipenser fulvescens)
Multiple molecular approaches yield no evidence for sex-determining genes in lake sturgeon (Acipenser fulvescens)
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DOI：
10.1111/j.1439-0426.2008.01156.x
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发表时间：
2008-12-01
影响因子：
0.9
通讯作者：
DeWoody, J. A.
中科院分区：
文献类型：
--
作者：
McCormick, C. R.;Bos, D. H.;DeWoody, J. A.
常见的基于DNA的性别鉴定方法已广泛用于哺乳动物和鸟类的保护和管理。然而，许多鱼类没有遗传性别决定，而在那些有性别决定的鱼类中，所涉及的基因的可塑性意味着通常需要进行物种特异性测定。这种DNA性别标记将是特别有价值的湖鲟鱼（鲟），因为他们的性单态，延迟性成熟，和保护地位。我们试图用几种不同的分子遗传学方法，包括随机扩增多态性DNA，代表性差异分析，消减杂交，和候选基因的方法，以确定男性和女性湖鲟之间的遗传差异。最终，一些基因被鉴定出来，但没有一个是性别特异性的。虽然性别决定的最终机制尚不清楚，但湖鲟的性别决定可能是环境因素，特别是最近的研究也未能在其他鲟鱼物种中确定性别决定基因。
Common DNA-based sexing assays have been widely used for the conservation and management of mammals and birds. However, many fishes do not have genetic sex determination and in those that do, the plasticity of the genes involved means that species-specific assays are normally required. Such DNA-sexing markers would be especially valuable in lake sturgeon (Acipenser fulvescens) because of their sexual monomorphism, delayed sexual maturity, and conservation status. We tried to identify genetic differences between male and female lake sturgeon using several different molecular genetic methods, including randomly amplified polymorphic DNA, representational difference analyses, subtractive hybridization, and a candidate gene approach. Ultimately, a number of genes were identified but none was sex-specific. Although the ultimate mechanism of sex determination is yet unknown, it is possible that sex determination is environmental in lake sturgeon, especially since recent studies have also failed to identify sex determination genes in other sturgeon species.