Retinal ganglion cells in normal hamsters and hamsters with novel retinal projections. I. Number, distribution, and size.
Retinal ganglion cells in normal hamsters and hamsters with novel retinal projections. I. Number, distribution, and size.
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正常仓鼠和具有新视网膜投射的仓鼠的视网膜神经节细胞。
DOI：
10.1002/cne.903530203
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Frost,DO
中科院分区：
文献类型：
--
作者：
Metin,C;Irons,WA;Frost,DO
我们研究了正常叙利亚仓鼠和视网膜投射至丘脑听觉和体感核团的仓鼠视网膜神经节细胞的数量、空间分布和大小。辣根过氧化物酶逆行填充显示，正常成年仓鼠视网膜内约有64600个对侧投射的视网膜神经节细胞(RGC)和1700个同侧投射的RGC。对侧投射的RGC分布于整个视网膜，并有两个局部密度峰位于高RGC密度的中心条纹内，该条纹大致沿鼻-颞轴定向。RGC密度落在中央条纹的上方和下方，并向上视网膜倾斜。单侧投射的视网膜节细胞弥漫分布于新月体内，位于新月体的内缘。对侧投射的视网膜节细胞胞体直径为6~25μm，呈单峰分布，峰值在10~13μm范围内，偏向较小的值，尾长向较大的值倾斜。在密度较高的区域，对侧投射的视网膜节细胞往往较小。无论是在全球还是在新月体内，单侧投射的视网膜节细胞往往比对侧投射的视网膜节细胞大，其大小分布不那么规则，与局部密度的相关性也较差。新生仓鼠的视网膜向听觉和躯体感觉系统投射新的视网膜，其视网膜投射的视网膜节细胞数量约为正常数量的四分之一，尽管视网膜节细胞的绝对密度急剧下降，但其相对密度分布接近正常。正常仓鼠和新生仓鼠的RGC胞体直径的范围和分布相似，在正常仓鼠中，对侧投射的RGC胞体在密度较高的区域往往较小。我们在正常仓鼠的结果表明，视网膜内机制在RGC大小的确定中发挥了作用。我们在新生儿手术仓鼠中的发现表明，尽管这些动物的RGC数量减少，但在正常和新生儿手术仓鼠的视网膜中发现了相同类型的RGC。©1995 Wiley-Liss公司
We examined the number, spatial distribution, and size of ganglion cells in the retinae of normal Syrian hamsters and hamsters with retinal projections to the auditory and somatosensory nuclei of the thalamus, induced by neonatal surgery. As revealed by retrograde filling with horseradish peroxidase, there are about 64,600 contralaterally projecting retinal ganglion cells (RGCs) and 1,700 ipsilaterally projecting RGCs in the retinae of normal adult hamsters. Contralaterally projecting RGCs are distributed throughout the retina and have two local density peaks located within a central streak of high RGC density that is oriented approximately along the nasal‐temporal axis. RGC density falls above and below the central streak, with a steeper gradient towards the upper retina. Ipsilaterally projecting RGCs are diffusely distributed within a crescent at the inferotemporal retinal periphery and are most dense at the internal border of the crescent. The soma diameter of contralaterally projecting RGCs ranges from 6 to 25 μm; the diameter distribution is unimodal, with a peak in the 10–13 μm range and is skewed toward smaller values, with an elongated tail towards higher values. Contralaterally projecting RGCs tend to be smaller in regions of higher density. Ipsilaterally projecting RGCs tend to be larger than contralaterally projecting RGCs both globally and within the temporal crescent, and their size distributions tend to be less regular and less well related to local density.The retinae of neonatally operated hamsters with novel retinal projections to the auditory and somatosensory systems contain about one‐fourth the normal number of contralaterally projecting RGCs, whose relative density distribution is approximately normal despite the drastic reduction of absolute RGC density. The range and distribution of RGC soma diameters are similar in normal and neonatally operated hamsters, and, in operated as in normal hamsters, contralaterally projecting RGC somata tend to be smaller in regions of higher density.Our results in normal hamsters suggest a role for intraretinal mechanisms in the determination of RGC size. Our findings in neonatally operated hamsters suggest that, despite the reduced number of RGCs in these animals, the same types of RGCs are found in the retinae of normal and neonatally operated hamsters. © 1995 Wiley‐Liss, Inc.