Leukotriene B4 mobilizes calcium without the breakdown of polyphosphoinositides and the production of phosphatidic acid in rabbit neutrophils.
Leukotriene B4 mobilizes calcium without the breakdown of polyphosphoinositides and the production of phosphatidic acid in rabbit neutrophils.
复制标题
白三烯 B4 动员钙，但不会分解多磷酸肌醇，也不在兔中性粒细胞中产生磷脂酸。
DOI：
10.1073/pnas.81.19.5966
复制
发表时间：
1984
影响因子：
11.1
通讯作者：
Sha'afi,RI
中科院分区：
文献类型：
--
作者：
Volpi,M;Yassin,R;Tao,W;Molski,TF;Naccache,PH;Sha'afi,RI
通过荧光染料quin-2测定，在兔中性粒细胞中加入fMet-Leu-Phe、白三烯B4或花生四烯酸会导致细胞内游离钙水平升高。钙反应快速且呈剂量依赖性，白三烯B4的ED50为0.12 +/- 0.05 nM， fMet-Leu-Phe的ED50为0.20 +/- 0.02 nM，花生四烯酸的ED50为320 +/- 30 nM。然而，与fMet-Leu-Phe不同，白三烯B4在高达70 nM的浓度下不会导致任何磷酸肌苷的显著分解或产生磷脂酸、花生四烯酸或1,2-二酰基甘油。花生四烯酸的添加使磷脂酸的产量很少（不到20%）。此外，与钙动员相比，fMet-Leu-Phe生成磷脂酸的剂量-反应曲线向右偏移。本文报道的结果表明，尽管在许多方面相似，但这两种趋化因子的作用机制存在重要的质的差异。他们还表明白三烯B4的钙动员不是由任何磷酸肌苷的分解介导的，白三烯B4、花生四烯酸和可能的fmet -亮氨酸的钙动员不是由磷脂酸的产生介导的。
The addition of fMet-Leu-Phe, leukotriene B4, or arachidonic acid to rabbit neutrophils causes a rise in the level of intracellular free calcium as measured by the fluorescent dye quin-2. The calcium response is rapid and dose-dependent with an ED50 of 0.12 +/- 0.05 nM for leukotriene B4, 0.20 +/- 0.02 nM for fMet-Leu-Phe, and 320 +/- 30 nM for arachidonic acid. However, unlike fMet-Leu-Phe, leukotriene B4 at concentrations up to 70 nM does not cause a significant breakdown of any of the phosphoinositides or the generation of phosphatidic acid, arachidonic acid, or 1,2-diacylglycerol. The addition of arachidonic acid causes little (less than 20%) production of phosphatidic acid. Furthermore, the dose-response curve of the generation of phosphatidic acid by fMet-Leu-Phe is shifted to the right when compared with that for calcium mobilization. The results reported here indicate that, although similar in many respects, there are important qualitative differences between the mechanisms of action of these two chemotactic factors. They also show that calcium mobilization by leukotriene B4 is not mediated by the breakdown of any of the phosphoinositides, and calcium mobilization by leukotriene B4, arachidonic acid, and possibly fMet-Leu-Phe is not mediated by the generation of phosphatidic acid.