The prominent alteration in transcriptome and metabolome of Mycobacterium bovis BCG str. Tokyo 172 induced by vitamin B-1
The prominent alteration in transcriptome and metabolome of Mycobacterium bovis BCG str. Tokyo 172 induced by vitamin B-1
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牛分枝杆菌 BCG str 转录组和代谢组的显着变化。
DOI：
10.1186/s12866-019-1492-9
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发表时间：
2019
期刊：
影响因子：
4.2
通讯作者：
Liu Siguo
中科院分区：
文献类型：
--
作者：
Song Ningning;Li Zhaoli;Cui Ziyin;Chen Liping;Cui Yingying;Dang Guanghui;Li Zhe;Li He;Liu Siguo
维生素B1（VB 1）是一种重要的膳食营养素，也是调节细胞和代谢过程中几种关键酶的必需辅助因子，更重要的是激活免疫系统。迄今为止，VB 1在结核分枝杆菌中的确切作用仍有待充分了解。在这项研究中，转录和代谢的VB 1处理的分枝杆菌。通过RNA测序和LC-MS（液相色谱-质谱联用）分析牛BCG。BCG菌株的选择是基于其与M.结核细胞生长试验结果表明，VB 1在体外抑制BCG的生长速率。转录组学分析显示，与脂肪酸代谢、胆固醇代谢、糖脂代谢、DNA复制、蛋白质翻译、细胞分裂和细胞壁形成相关的基因在M.用VB 1处理牛BCG。此外，代谢组学LC-MS数据表明，大部分氨基酸和二磷酸腺苷（ADP）在M。Bovis BCG strain.本研究为了解VB 1对卡介苗的影响提供了分子和代谢基础。
Vitamin B1 (VB1) is a crucial dietary nutrient and essential cofactor for several key enzymes in the regulation of cellular and metabolic processes, and more importantly in the activation of immune system. To date, the precise role of VB1 in Mycobacterium tuberculosis remains to be fully understood. In this study, the transcriptional and metabolic profiles of VB1-treated Mycobacterium. bovis BCG were analyzed by RNA-sequencing and LC-MS (Liquid chromatography coupled to mass spectrometry). The selection of BCG strain was based on its common physiological features shared with M. tuberculosis. The results of cell growth assays demonstrated that VB1 inhibited the BCG growth rate in vitro. Transcriptomic analysis revealed that the expression levels of genes related to fatty acid metabolism, cholesterol metabolism, glycolipid catabolism, DNA replication, protein translation, cell division and cell wall formation were significantly downregulated in M. bovis BCG treated with VB1. In addition, the metabolomics LC-MS data indicated that most of the amino acids and adenosine diphosphate (ADP) were decreased in M. bovis BCG strain after VB1 treatment. This study provides the molecular and metabolic bases to understand the impacts of VB1 on M.bovis BCG.