Electroacupuncture Improves Neurobehavioral Function Through Targeting of SOX2-Mediated Axonal Regeneration by MicroRNA-132 After Ischemic Stroke
Electroacupuncture Improves Neurobehavioral Function Through Targeting of SOX2-Mediated Axonal Regeneration by MicroRNA-132 After Ischemic Stroke
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DOI：
10.3389/fnmol.2018.00471
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发表时间：
2018-12
影响因子：
4.8
通讯作者：
Xiaoying Zhao;Fuhai Bai;Erfei Zhang;Dandan Zhou;Tao Jiang;Heng Zhou;Qiang Wang
中科院分区：
文献类型：
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作者：
Xiaoying Zhao;Fuhai Bai;Erfei Zhang;Dandan Zhou;Tao Jiang;Heng Zhou;Qiang Wang
本课题组前期研究表明，电针可促进缺血性脑卒中后神经行为功能的恢复，但其机制尚不清楚。microRNA（miRNAs）在大脑中含量丰富，参与转录后基因调控。在脑缺血再灌注过程中，miRNAs在中枢神经系统中执行与受损神经的再生和修复相关的许多生物学功能。我们前期的研究也表明，大脑中动脉闭塞（MCAO）后，脑组织中的miRNA-132（miR-132）表达明显下调，电针可上调其表达。本研究旨在确定电针上调miR-132是否能改善脑卒中后受损的神经，并筛选miR-132的潜在靶点。结果表明，电针可上调miR-132，抑制SOX 2的表达，明显改善MCAO后神经行为功能的恢复。此外，我们的结果还表明，上调的miR-132抑制了氧糖剥夺（OGD）后原代神经元中的SOX 2，从而促进了神经突的生长。总之，电针通过靶向miR-132介导的SOX 2介导的轴突再生来增强缺血性卒中的神经行为功能恢复。
Our previous studies have shown that electroacupuncture (EA) enhances neurobehavioral functional recovery after ischemic stroke, however, the underlying regulatory mechanisms remain unclear. MicroRNAs (miRNAs) are abundant in the brain and are involved in post-transcriptional gene regulation. During cerebral ischemia reperfusion, miRNAs perform numerous biological functions in the central nervous system related to regeneration and repair of damaged nerves. Our previous studies also have shown that the expression of miRNA-132 (miR-132) is obviously down-regulated after stroke by middle cerebral artery occlusion (MCAO), which can be up-regulated by EA. This study aimed to identify whether up-regulation of miR-132 by EA improved the damaged nerves after stroke and to screen the potential target of miR-132. The results showed that EA up-regulated miR-132 thus suppressing SOX2 expression in vivo after MCAO, which obviously ameliorated neurobehavioral functional recovery. Moreover, our results also suggested that up-regulated miR-132 suppressed SOX2 in primary neurons after oxygen-glucose deprivation (OGD), which promoted neurite outgrowth. In conclusion, EA enhances neurobehavioral functional recovery against ischemic stroke through targeting of SOX2-mediated axonal regeneration by miR-132.