Differential Responses Between Monocytes and Monocyte-Derived Macrophages for Lipopolysaccharide Stimulation of Calves
Differential Responses Between Monocytes and Monocyte-Derived Macrophages for Lipopolysaccharide Stimulation of Calves
复制标题
单核细胞和单核细胞源性巨噬细胞对脂多糖刺激犊牛的差异反应
DOI：
10.1038/cmi.2009.30
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发表时间：
2009-06-01
影响因子：
24.1
通讯作者：
Yamaguchi, Takahiro
中科院分区：
文献类型：
--
作者：
Guo, Yijie;Zhao, Guoqi;Yamaguchi, Takahiro
在本实验中，检查了脂多糖（LPS）刺激的单核细胞和单核细胞衍生的巨噬细胞中Toll样受体的表达模式。从4头日本小牛中采集颈静脉血，分离外周血单核细胞（PBMC）。将细胞直接用于通过磁性细胞分选收集单核细胞或在Repcell中培养7天以收集单核细胞衍生的巨噬细胞。然后分析LPS刺激24 h后单核细胞和单核细胞源性巨噬细胞中TLRs和细胞因子的mRNA表达模式。LPS刺激单核细胞和单核细胞衍生的巨噬细胞导致TNF-α、IL-6和IL-8的mRNA转录水平增加。LPS刺激后，单核细胞源性巨噬细胞TNF-α和IL-6 mRNA的表达显著增加。LPS刺激单核细胞后TLRs mRNA表达无明显变化，而单核细胞源性巨噬细胞TLRs mRNA表达则较为复杂。LPS刺激后，TLR 1、3、5、8和10的mRNA表达明显降低，而TLR 2、4、6和7的mRNA表达与对照组相比无显著性差异。单核细胞和单核细胞源性巨噬细胞作为抗原提呈细胞，对LPS的反应不同，可能在天然免疫系统中具有不同的功能。
In this experiment Toll-like receptor expression pattern in monocytes and monocyte-derived macrophages by lipopolysaccharide (LPS) stimulation was examined. Jugular venous blood was collected from four Japanese calves, and the peripheral blood mononuclear cells (PBMCs) were isolated. The cells were directly used for collecting monocytes by magnetic cell sorting or cultured for 7 days to collect monocyte-derived macrophages in Repcell. Then we analyzed the mRNA expression pattern of TLRs and cytokines in monocytes and monocyte-derived macrophages after LPS stimulation for 24 h. LPS stimulation of both monocytes and monocyte-derived macrophages resulted in an increase in the levels of mRNA transcripts for TNF-α, IL-6 and IL-8. Moreover, TNF-α and IL-6 mRNA expressions were significantly augmented by LPS stimulation in monocyte-derived macrophages. TLRs mRNA expressions were unchanged after LPS stimulation of monocytes, while TLRs mRNA expressions in monocyte-derived macrophages were complicated. TLR1, 3, 5, 8 and 10 were significantly decreased after LPS stimulation and there were no differences in the mRNA expressions of TLR2, 4, 6 and 7 between the groups of control and LPS stimulation. Besides, no expression of TLR9 was found. As antigen presenting cells, monocytes and monocyte-derived macrophages respond differently to LPS, so they may have different functions in the innate immune system.