Lacticaseibacillus casei CNRZ1874 supplementation promotes M1 alveolar macrophage activation and attenuates Mycoplasma pneumoniae pneumonia
Lacticaseibacillus casei CNRZ1874 supplementation promotes M1 alveolar macrophage activation and attenuates Mycoplasma pneumoniae pneumonia
复制标题
DOI：
10.1093/jambio/lxad022
复制
发表时间：
2023-03-01
影响因子：
4
通讯作者：
Zhu，Cuiming
中科院分区：
文献类型：
--
作者：
Zhang，Naiyu;Zeng，Wuwei;Zhu，Cuiming
目的探讨肠道补充干酪乳杆菌CNRZ 1874对C57 BL/6 J小鼠肺炎支原体感染的保护作用，为临床治疗肺炎支原体感染提供理论依据。方法和结果C57 BL/6 J小鼠灌胃L. caseiCNRZ 1874或PBS连续感染7天后，再用M.肺炎第8天。L. caseiCNRZ 1874显著降低M.感染后第3、10天，肺内的肺炎负荷减轻，肺部炎症减轻。重要的是，口服L. caseiCNRZ 1874促进M1肺泡巨噬细胞活化，表现为iNOS、TNF-α和CXCL 1表达增加，而抑制M2肺泡巨噬细胞活化，表现为Arg 1和Chi 313表达显著降低。与M1肺泡巨噬细胞活化和支原体清除增强一致，L. caseiCNRZ 1874组与对照组比较。然而，口服L. caseiCNRZ 1874可能不影响M.肺炎克雷伯氏菌（M.结论肠道补充L. caseiCNRZ 1874可促进M1肺泡巨噬细胞活化，从而有助于M. pneumoniae和M. pneumoniaepneumoniae的减毒。
AimsTo evaluate the protective effect of intestinal supplementation withLacticaseibacillus caseiCNRZ1874 on the inflammatory response induced byMycoplasma pneumoniaein C57BL/6 J mice, and provide a potential strategy for alleviatingM. pneumoniaepneumonia.Methods and resultsC57BL/6 J mice were gavaged withL. caseiCNRZ1874 or PBS for 7 consecutive days, and then infected withM. pneumoniaeon day 8. Treatment withL. caseiCNRZ1874 significantly reducedM. pneumoniaeloads in the lungs and alleviated the lung inflammation on day 3 and 10 after pathogen infection. Importantly, oral administration withL. caseiCNRZ1874 promoted M1 alveolar macrophages activation as evidenced by increased expression of iNOS, TNF-α, and CXCL1, while inhibited M2 alveolar macrophages activation as the expression of Arg1 and Chi3l3 were significantly decreased. In consistent with the M1 alveolar macrophages activation and enhanced mycoplasma clearance, the level of TNF-α was increased while the level of IL-4 was decreased in lung tissue from theL. caseiCNRZ1874 group compared with the control group. However, oral administration withL. caseiCNRZ1874 may not influence adaptive immunity induced byM. pneumoniaeas evaluated byM. pneumoniaespecific antibodies and T cells responses in spleen.ConclusionsIntestinal supplementation withL. caseiCNRZ1874 can promote M1 alveolar macrophages activation, which contributes to the clearance ofM. pneumoniaeand attenuation ofM.pneumoniaepneumonia.