The length of BTV-8 viraemia in cattle according to infection doses and diagnostic techniques
The length of BTV-8 viraemia in cattle according to infection doses and diagnostic techniques
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DOI：
10.1016/j.rvsc.2010.12.017
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发表时间：
2011-10-01
影响因子：
2.4
通讯作者：
Savini, G.
中科院分区：
文献类型：
--
作者：
Di Gialleonardo, L.;Migliaccio, P.;Savini, G.
使用四组无BTV的弗里斯兰和杂交小牛来确定用不同剂量的BTV-8意大利分离株感染后病毒血症的持续时间。第一组5只动物感染10 TCID 50的BTV-8，第二组4只动物感染10（3）TCID 50，第三组（也包括4只动物）感染10（6）TCID 50。向第四组的四只动物给予含有未感染组织培养基的安慰剂。通过真实的时间RTPCR和病毒分离评估病毒血症。在所有感染组中，病毒分离能够在感染后39天（dpi）内检测到感染性病毒，而RT-PCR在151-157 dpi内呈阳性。感染剂量不影响BTV-8病毒血症的长度和模式，并证实真实的时间RT-PCR仍然是阳性的，虽然没有循环病毒在外周循环中检测。(C)2011爱思唯尔有限公司保留所有权利。
Four groups of BTV free Frisian and cross bred calves were used to determine the length of viraemia following infection with different doses of BTV-8 Italian isolate. The first group of five animals was infected with 10 TCID50 of BTV-8, the second group of four animals with 10(3) TCID50 and the third group, which also included four animals, was infected with 10(6) TCID50. A placebo containing uninfected tissue culture medium was given to the four animals of the fourth group. The viraemia was evaluated by real time RTPCR and virus isolation. In all infected groups, virus isolation was able to detect infectious virus up to 39 days post infection (dpi) while RT-PCR was positive up to 151-157 dpi. Infectious dose did influence neither the length nor the pattern of BTV-8 viraemia and confirmed that real time RT-PCR remains positive although no circulating virus is detectable in the peripheral circulation. (C) 2011 Elsevier Ltd. All rights reserved.