Multidimensional Analysis of Nanoparticles with Highly Disperse Properties Using Multiwavelength Analytical Ultracentrifugation
Multidimensional Analysis of Nanoparticles with Highly Disperse Properties Using Multiwavelength Analytical Ultracentrifugation
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DOI：
10.1021/nn503205k
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发表时间：
2014-09-01
期刊：
影响因子：
17.1
通讯作者：
Coelfen, Helmut
中科院分区：
文献类型：
--
作者：
Walter, Johannes;Loehr, Konrad;Coelfen, Helmut
纳米颗粒技术日益复杂的全球趋势必须与适用于科学和技术中许多不同颗粒系统的更先进的尺寸，形状和相关性质的表征方法直接相关。用于纳米颗粒表征的可用技术主要集中于尺寸表征。然而，同时进行尺寸和形状表征仍然是一个尚未解决的重大挑战。我们证明，分析超离心与多波长检测器是一个强大的技术，以解决多维纳米粒子分析。使用高性能的光学装置和数据采集软件，在一个单一的实验中，以无与伦比的精度和分辨率获得了有关尺寸，形状各向异性和光学特性的信息。动态转子速度梯度使我们能够在短时间尺度上研究广泛的分布，并区分金纳米棒物种，包括对聚集体形成的精确评估。我们报告如何区分不同种类的单壁碳纳米管在一个实验中使用波长依赖的沉降系数分布，而无需耗时的纯化方法。此外，不同尺寸和光学性质的CdTe纳米粒子进行了研究，在一个单一的实验提供重要的信息的结构-性能关系。因此，纳米颗粒系统的尺寸，密度，形状和光学性质的多维信息变得可通过配备多波长检测的分析超离心。
The worldwide trend in nanoparticle technology toward increasing complexity must be directly linked to more advanced characterization methods of size, shape and related properties, applicable to many different particle systems in science and technology. Available techniques for nanoparticle characterization are predominantly focused on size characterization. However, simultaneous size and shape characterization is still an unresolved major challenge. We demonstrate that analytical ultracentrifugation with a multiwavelength detector is a powerful technique to address multidimensional nanoparticle analysis. Using a high performance optical setup and data acquisition software, information on size, shape anisotropy and optical properties were accessible in one single experiment with unmatched accuracy and resolution. A dynamic rotor speed gradient allowed us to investigate broad distributions on a short time scale and differentiate between gold nanorod species including the precise evaluation of aggregate formation. We report how to distinguish between different species of single-wall carbon nanotubes in just one experiment using the wavelength-dependent sedimentation coefficient distribution without the necessity of time-consuming purification methods. Furthermore, CdTe nanoparticles of different size and optical properties were investigated in a single experiment providing important information on structure-property relations. Thus, multidimensional information on size, density, shape and optical properties of nanoparticulate systems becomes accessible by means of analytical ultracentrifugation equipped with multiwavelength detection.