Mediterranean meal versus Western meal effects on postprandial ox-LDL, oxidative and inflammatory gene expression in healthy subjects: a randomized controlled trial for nutrigenomic approach in cardiometabolic risk
Mediterranean meal versus Western meal effects on postprandial ox-LDL, oxidative and inflammatory gene expression in healthy subjects: a randomized controlled trial for nutrigenomic approach in cardiometabolic risk
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DOI：
10.1007/s00592-016-0917-2
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发表时间：
2017-02-01
影响因子：
3.8
通讯作者：
Di Renzo, Laura
中科院分区：
文献类型：
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作者：
De Lorenzo, Antonino;Bernardini, Sergio;Di Renzo, Laura
炎症和氧化损伤对心血管疾病（CVD）的发展有重要作用。餐后氧化应激和炎症的特征在于在食用富含脂质和/或碳水化合物的膳食后生物体对氧化损伤的易感性增加。营养素调节免疫系统，并通过降低氧化低密度脂蛋白（ox-LDL）水平发挥保护作用。本研究的目的是评估富含生育酚的地中海餐（TEM）和西方高脂餐（HFM）后餐后血浆ox-LDL水平和13个与氧化应激（HOSp）和人类炎性小体通路（HIp）相关的基因表达。此外，还计算了地中海风味指数来定义两餐的质量。采用酶联免疫吸附法测定Ox-LDL水平，采用qRT-PCR测定HOSp和HIp相关的13个基因的基因表达，HFM与TEM相比，Ox-LDL水平显着降低（p < 0.05）。每次饮食处理后显着过表达基因的百分比如下：（A）基线与HFM：7.69%HIp和23.08%HOSp;（B）基线与TEM：7.69%HIp和7.69%HOSp;（C）HFM与TEM：TEM能降低餐后心血管危险因素ox-LDL及炎症和氧化应激相关基因的表达。在确定之前，需要对更大人群进行长期研究。conclusions.ClinicalTrials.gov
Inflammation and oxidative damage contribute significantly to the development of cardiovascular diseases (CVD). Postprandial oxidative stress and inflammation are characterized by an increased susceptibility of the organism toward oxidative damage after consumption of a meal rich in lipids and/or carbohydrates. Micronutrients modulate immune system and exert a protective action by reducing oxidized low-density lipoprotein (ox-LDL) level. The aim of the present study was to evaluate the postprandial plasma ox-LDL level and the gene expression of 13 genes related to oxidative stress (HOSp) and human inflammasome pathways (HIp), after a tocopherol-enriched Mediterranean meal (TEM), and a Western high-fat meal (HFM). Moreover, Mediterranean Adequacy Index was calculated to define the quality of both meals.We set up a randomized and crossover trial in healthy human volunteers. Ox-LDL level was measured by enzyme-linked immunosorbent assay and the gene expression of 13 genes related to HOSp and HIp by qRT-PCR.Ox-LDL levels significantly decreased comparing HFM versus TEM (p < 0.05). Percentages of significantly overexpressed genes after each dietary treatment are as follows: (A) baseline versus HFM: 7.69 % HIp and 23.08 % HOSp; (B) baseline versus TEM: 7.69 % HIp and 7.69 % HOSp; (C) HFM versus TEM: 15.38 % HIp and 15.38 % HOSp.TEM reduced postprandial risk factors of CVD, such as ox-LDL, and the expression of inflammation and oxidative stress-related genes. Chronic studies on larger population are necessary before definitive conclusions.ClinicalTrials.gov Id: NCT01890070.