Dietary Ribonucleic Acid Suppresses Inflammation of Adipose Tissue and Improves Glucose Intolerance That Is Mediated by Immune Cells in C57BL/6 Mice Fed a High-Fat Diet
Dietary Ribonucleic Acid Suppresses Inflammation of Adipose Tissue and Improves Glucose Intolerance That Is Mediated by Immune Cells in C57BL/6 Mice Fed a High-Fat Diet
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DOI：
10.3177/jnsv.61.73
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发表时间：
2015-02-01
影响因子：
1.6
通讯作者：
Shuto, Emi
中科院分区：
文献类型：
--
作者：
Sakai, Tohru;Taki, Tomoyo;Shuto, Emi
最近的证据表明，免疫细胞在脂肪组织中炎性巨噬细胞的分化中发挥重要作用，从而导致全身慢性炎症。膳食核糖核酸（RNA）已被证明可以调节免疫功能。我们假设RNA影响脂肪组织的免疫细胞功能，进而改善脂肪组织的炎症反应。以C57BL/6小鼠为背景的C57/BL6小鼠和重组激活基因1 （rag1）敲除小鼠饲喂含有1% RNA的高脂饲料12周。进行口服葡萄糖耐量试验。饲喂高脂饲料的C57BL/6小鼠在添加RNA后，口服葡萄糖后曲线下面积（AUC）小于对照组。饲粮中添加RNA可降低脂肪组织中炎症相关细胞因子mRNA表达水平和血清白细胞介素-6水平。有趣的是，在T和B细胞缺陷的RAG-1敲除小鼠中，通过补充RNA降低的AUC值被消除。这些结果表明，RNA以T和B细胞依赖的方式改善脂肪组织的炎症并降低口服葡萄糖给药后的AUC值。
Recent evidence suggests that immune cells play an important role in differentiation of inflammatory macrophages in adipose tissue, which contributes to systemic chronic inflammation. Dietary ribonucleic acid (RNA) has been shown to modulate immune function. We hypothesized that RNA affects immune cell function in adipose tissue and then improves inflammatory response in adipose tissue. C57/BL6 mice and recombination activating gene-1 (RAG-1) knockout mice on a C57BL/6 mice background were fed a high-fat diet containing 1% RNA for 12 wk. An oral glucose tolerance test was performed. Supplementation of dietary RNA in C57BL/6 mice fed a high-fat diet resulted in a smaller area under the curve (AUC) after oral glucose administration than that for control mice. The mRNA expression levels of inflammation-related cytokines in adipose tissue and serum interleukin-6 levels were reduced by dietary RNA supplementation. Interestingly, reduction of the AUC value by RNA supplementation was abolished in T and B cell-deficient RAG-1 knockout mice. These results indicate that RNA improves inflammation in adipose tissue and reduces the AUC value following oral glucose administration in a T and B cell-dependent manner.