kin-3 genetically suppresses sur-6 in centrosome assembly during Caenorhabditis elegans embryogenesis.
kin-3 genetically suppresses sur-6 in centrosome assembly during Caenorhabditis elegans embryogenesis.
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DOI：
10.17912/micropub.biology.000791
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发表时间：
2023
影响因子：
--
通讯作者：
Song, Mi Hye
中科院分区：
文献类型：
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作者：
Medley, Jeffrey C;Song, Mi Hye
蛋白质磷酸化在细胞周期进程中起着至关重要的作用。在秀丽线虫中，酪蛋白激酶II(CK2)负向调节中心体组装，而蛋白磷酸酶2A(PP2A)SuR-6/B55对中心体复制起正调节作用，提示CK2和PP2A Sur-6/B55在中心体组装中扮演相反的角色。在这里，我们研究了编码CK2催化亚基的KIN-3和编码PP2A调节亚单位SuR-6/B55的SuR-6之间的遗传相互作用。我们的结果表明，KIN-3(RNAi)部分恢复了正常的中心体复制和胚胎活力，提示KIN-3在秀丽线虫胚胎发生过程中从基因上抑制了中心体组装中的SUR-6。
Protein phosphorylation plays a critical role in cell cycle progression. In Caenorhabditis elegans , Casein Kinase II (CK2) negatively regulates centrosome assembly, and Protein Phosphatase 2A (PP2A) SUR-6 /B55 acts as a positive regulator of centrosome duplication, suggesting CK2 and PP2A SUR-6 /B55 play opposing roles in centrosome assembly. Here, we examined the genetic interaction between kin-3 , encoding the catalytic subunit of CK2, and sur-6 , encoding the PP2Aregulatory subunit SUR-6 /B55, in Caenorhabditis elegans embryos. Our results show that kin-3 (RNAi) partially restores normal centrosome duplication and embryonic viability to hypomorphic sur-6 mutants, suggesting that kin-3 genetically suppresses sur-6 in centrosome assembly during Caenorhabditis elegans embryogenesis.