Brevinin-1 and multiple insulin-releasing peptides in the skin of the frog Rana palustris
Brevinin-1 and multiple insulin-releasing peptides in the skin of the frog Rana palustris
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DOI：
10.1677/joe.0.1810347
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发表时间：
2004-05-01
影响因子：
4
通讯作者：
Abdel-Wahab, YHA
中科院分区：
文献类型：
--
作者：
Marenah, L;Flatt, PR;Abdel-Wahab, YHA
很少有研究全面检查两栖类颗粒腺分泌物的新型促胰岛素肽。本研究涉及从沼泽蛙皮肤分泌物中分离和表征生物活性肽。通过温和的电刺激从背部皮肤表面获得的粗分泌物通过反相HPLC在半制备型Vaporac C18柱上纯化，得到80个级分。使用葡萄糖响应性BRIN-BD 11细胞测定这些级分的胰岛素释放活性。在不存在（对照）和存在各种组分的情况下，在补充有5-6 mmol/l葡萄糖的Krebs Ringer碳酸氢盐缓冲液中进行急性20 min孵育。组分29-54和组分68-75显示出显著的2-0-6-5倍的胰岛素释放活性增加（P
Few studies have comprehensively examined amphibian granular gland secretions for novel insulinotropic peptides. This study involved isolation and characterisation of biologically active peptides from the skin secretions of Rana palustris frogs. Crude secretions obtained by mild electrical Stimulation from the dorsal skin surface were purified by reversed-phase HPLC on a semipreparative Vydac C18 column, yielding 80 fractions. These fractions were assayed for insulin-releasing activity using glucose-responsive BRIN-BD11 cells. Acute 20 min incubations were performed in Krebs Ringer bicarbonate buffer supplemented with 5-6 mmol/l glucose in the absence (control) and presence of various fractions. Fractions 29-54 and fractions 68-75 showed significant 2-0-6-5-fold increases in insulin-releasing activity (P