PC-based high-speed video-oculography for measuring rapid eye movements in mice
PC-based high-speed video-oculography for measuring rapid eye movements in mice
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DOI：
10.1016/j.neures.2004.02.002
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发表时间：
2004-05-01
影响因子：
2.9
通讯作者：
Isa, T
中科院分区：
文献类型：
--
作者：
Sakatani, T;Isa, T
我们新开发了一种红外视频视觉系统，用于在线跟踪清醒和头部固定小鼠的眼睛位置，具有高时间分辨率（240 Hz）。该系统由市售的高速CCD摄像机和在IBM-PC上运行的LabVIEW编写的图像处理软件以及外挂的视频采集板组成。该软件通过将圆形函数拟合到瞳孔边界来计算瞳孔的中心和面积，并允许对像老鼠这样的小动物的眼睛位置进行稳健和稳定的跟踪。即使瞳孔的一部分被阴影覆盖或被眼睑或角膜反射遮挡，也可以进行在线计算以获得合理的圆形拟合瞳孔边界。基于眼球解剖模型，通过估计眼球的旋转中心，将瞳孔在二维视频平面上的位置转换为眼球的旋转角度。通过这个记录系统，可以对老鼠的快速眼球运动进行定量分析，比如扫视。这将为利用不同的突变系分析动眼肌和认知功能的分子基础提供有力的工具。(C) 2004爱思唯尔爱尔兰有限公司和日本神经科学学会。版权所有。
We newly developed an infrared video-oculographic system for on-line tracking of the eye position in awake and head-fixed mice, with high temporal resolution (240 Hz). The system consists of a commercially available high-speed CCD camera and an image processing software written in LabVIEW run on IBM-PC with a plug-in video grabber board. This software calculates the center and area of the pupil by fitting circular function to the pupil boundary, and allows robust and stable tracking of the eye position in small animals like mice. On-line calculation is performed to obtain reasonable circular fitting of the pupil boundary even if a part of the pupil is covered with shadows or occluded by eyelids or corneal reflections. The pupil position in the 2-D video plane is converted to the rotation angle of the eyeball by estimating its rotation center based on the anatomical eyeball model. By this recording system, it is possible to perform quantitative analysis of rapid eye movements such as saccades in mice. This will provide a powerful tool for analyzing molecular basis of oculomotor and cognitive functions by using various lines of mutant mice. (C) 2004 Elsevier Ireland Ltd and The Japan Neuroscience Society. All rights reserved.