Cytotoxicity of Mycoplasma mycoides subsp mycoides small colony type to bovine epithelial cells
Cytotoxicity of Mycoplasma mycoides subsp mycoides small colony type to bovine epithelial cells
复制标题
DOI：
10.1128/iai.00938-07
复制
发表时间：
2008-01-01
影响因子：
3.1
通讯作者：
Frey, Joachim
中科院分区：
文献类型：
--
作者：
Bischof, Daniela F.;Janis, Carole;Frey, Joachim
不同菌株蕈状支原体亚种的细胞毒性。蕈状小集落型（SC）是传染性牛胸膜肺炎（CBPP）的病原体，使用胚胎小牛鼻上皮（ECaNEp）细胞在体外进行测量。从急性 CBPP 病例中分离出的菌株在甘油存在下诱导高细胞毒性，同时释放大量有毒的 H2O2，发现通过支原体菌株与宿主细胞的密切接触，这些有毒的 H2O2 易位到宿主细胞的细胞质中。目前使用的疫苗株还表现出高细胞毒性和高H2O2释放，表明它们的另一个毒力属性被减弱。从最近欧洲爆发的 CBPP 中分离出的菌株具有轻微的临床症状，其特点是甘油摄取系统缺陷，释放少量 H2O2，对 ECaNEp 细胞显示出低细胞毒性。蕈状支原体亚种蕈状SC菌株PG1释放大量H2O2，但仅有轻微的细胞毒性。 PG1 被发现与 ECaNEp 细胞结合的能力降低，并且无法将 H2O2 转移到牛细胞中，这与释放大量 H2O2 的强毒菌株相反。因此，H2O2有效转位到宿主细胞中是细胞毒作用的先决条件，并且需要完整的粘附机制以确保支原体与宿主细胞之间的紧密接触。
The cytotoxicities of various strains of Mycoplasma mycoides subsp. mycoides small colony type (SC), the agent of contagious bovine pleuropneumonia (CBPP), were measured in vitro using embryonic calf nasal epithellial (ECaNEp) cells. Strains isolated from acute cases of CBPP induced high cytotoxicity in the presence of glycerol, concomitant with the release of large amounts of toxic H2O2 that were found to be translocated into the cytoplasms of the host cells by close contact of the Mycoplasma strains with the host cells. Currently used vaccine strains also showed high cytotoxicity and high H2O2 release, indicating that they are attenuated in another virulence attribute. Strains isolated from recent European outbreaks of CBPP with mild clinical signs, which are characterized by a defect in the glycerol uptake system, released small amounts of H2O2 and showed low cytotoxicity to ECaNEp cells. M. mycoides subsp. mycoides SC strain PG1 released large amounts of H2O2 but was only slightly cytotoxic. PG1 was found to have a reduced capacity to bind to ECaNEp cells and was unable to translocate H2O2 into the bovine cells, in contrast to virulent strains that release large amounts of H2O2. Thus, an efficient translocation of H2O2 into host cells is a prerequisite for the cytotoxic effect and requires an intact adhesion mechanism to ensure a close contact between mycoplasmas and host cells.