Utilization of the serosal scarification model of postoperative intestinal adhesion formation to investigate potential adhesion-preventing substances in the rabbit.
Utilization of the serosal scarification model of postoperative intestinal adhesion formation to investigate potential adhesion-preventing substances in the rabbit.
复制标题
利用术后肠粘连形成的浆膜划痕模型研究兔体内潜在的防粘连物质。
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
P. Conlon
中科院分区：
文献类型：
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作者：
E. Singer;M. A. Livesey;I. Barker;M. Hurtig;P. Conlon
利用兔血清划痕模型比较了先前在围手术期给予接受探查性剖腹术的马的四种药物预防术后肠粘连发展的能力。比较的物质为32%右旋糖酐70（7 mL/kg）、1%羧甲基纤维素钠（7 mL/kg）、甲氧苄啶-磺胺嘧啶（30 mg/kg）和氟尼辛葡甲胺（1 mg/kg）。前两种在手术后腹腔内给药，后两种在围手术期全身给药。在未处理的浆膜划痕组中，所有动物均出现明显的纤维粘连。未给药对照组和任何给药组之间以及给药组之间的粘连动物数量均无显著差异。对尸检时收集的粘连进行显微镜检查，发现纤维与棉一致，周围有巨细胞反应和持续的急性炎症。纤维的来源可能是用于划破肠表面的棉剖腹海绵，因为肉芽肿和海绵纤维的图案在偏振光下看起来相似。虽然一致的肠粘连形成的兔子，异物肉芽肿的存在可能会阻止考虑这个模型用于未来的研究。在该模型中，测试的药物在预防术后小肠粘连形成方面无效。
A rabbit serosal scarification model was utilized to compare the ability of four drugs, previously administered peri-operatively to horses undergoing exploratory celiotomy, to prevent the development of postoperative intestinal adhesions. The substances compared were 32% Dextran 70 (7 mL/kg), 1% sodium carboxymethylcellulose (7 mL/kg), trimethoprim-sulfadiazine (30 mg/kg), and flunixin meglumine (1 mg/kg). The first two were administered intra-abdominally following surgery, while the latter two were administered systemically in the peri-operative period. Fibrous adhesions were evident in all animals in the untreated serosal scarification group. No significant difference in the number of animals with adhesions was found between the untreated control group and any treatment group, nor among the treatment groups. Microscopic examination of adhesions collected at postmortem examination revealed fibers consistent with cotton, surrounded by a giant-cell reaction and ongoing acute inflammation. The source of the fibers was likely the cotton laparotomy sponges used to scarify the intestinal surface, since the pattern in the granuloma and sponge fibers appeared similar under polarized light. Though consistent intestinal adhesion formation was produced in the rabbit, the presence of foreign body granulomas may prevent consideration of this model for future research. The drugs tested were ineffective in preventing the formation of postoperative small intestinal adhesions in this model.