EFFECT OF FERMENTATION SUGAR ON HYDROGEN-PEROXIDE ACCUMULATION BY STREPTOCOCCUS-LACTIS C10
EFFECT OF FERMENTATION SUGAR ON HYDROGEN-PEROXIDE ACCUMULATION BY STREPTOCOCCUS-LACTIS C10
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DOI：
10.1017/s0022029900032714
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发表时间：
1983-01-01
影响因子：
2.1
通讯作者：
CONDON, S
中科院分区：
文献类型：
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作者：
GRUFFERTY, RC;CONDON, S
过氧化氢积累在通气培养的S。乳酸C10，其量取决于所利用的糖。当能量来源是半乳糖或（在较小程度上）乳糖或麦芽糖时，H2O2达到自抑制水平。在葡萄糖的作用下，H_2O_2达到不引起明显抑制的水平，然后下降。洗涤的细胞悬浮液利用几种培养基成分，包括葡萄糖，半乳糖和酵母提取物作为O2摄取的底物，但无细胞提取物利用这些。支持无细胞提取物摄取O2的唯一底物是NADH。NADH氧化酶的活性不受碳水化合物代谢的几个中间产物的影响。获得的证据表明，它的合成调节通过诱导O2与压倒性的分解代谢产物阻遏型控制，当细胞生长的代价是葡萄糖。
Hydrogen peroxide accumulated in aerated cultures of S. lactis C10, the amount depending on the sugar being utilized. When the energy source was galactose or (to a lesser extent) lactose or maltose, H2O2 reached autoinhibitory levels. With glucose, H2O2 reached levels which did not cause obvious inhibition and then declined. Washed cell suspensions utilized several media ingredients including glucose, galactose and yeast extract as substrates for O2 uptake, but cell-free extracts utilized none of these. The only substrate which supported O2 uptake by cell-free extracts was NADH. The activity of NADH oxidase was not affected by several intermediates of carbohydrate metabolism. Evidence was obtained to show that its synthesis was regulated via induction by O2 with an overriding catabolite repression type control, when cells were grown at the expense of glucose.