miR-548c-5p inhibits proliferation and migration and promotes apoptosis in CD90+ HepG2 cells
miR-548c-5p inhibits proliferation and migration and promotes apoptosis in CD90+ HepG2 cells
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DOI：
10.2478/v10019-012-0025-z
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发表时间：
2012-09-01
影响因子：
2.4
通讯作者：
Yang, Guang-Shun
中科院分区：
文献类型：
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作者：
Fang, Lin;Zhang, Hai-Bing;Yang, Guang-Shun
背景自肿瘤干细胞（tumor stem cells，TSCs）理论提出以来，肝癌干细胞样细胞（liver cancer stem cell，LCSC）已成为肝癌研究的热点之一。本研究以CD 90（+）细胞作为可能的LCSC样细胞，分析CD 90（+）HepG 2细胞中miRNA及其基因的表达。初步研究表明miR-548 c-5 p对CD 90（+）HepG 2细胞具有潜在的作用，并随后用于进一步的研究。将CD 90（+）HepG 2细胞分为miR-548 c-5 p模拟转染组和对照组。MTT法检测CD 90（+）HepG 2细胞增殖情况。伤口愈合实验和transwell迁移实验检测细胞的迁移和侵袭能力。荧光显微镜下观察细胞凋亡情况。结果显示，在CD 90（+）HepG 2细胞中，caspase-3和bcl-2表达下调，caspase-8表达上调。miR-548 c-5 p转染CD 90（+）HepG 2细胞后，可下调beta-catenin、Tg 737、bcl-2、bcl-XL和caspase-3的表达，抑制细胞的增殖、迁移和侵袭，促进细胞凋亡。我们的研究结果表明，LCSC样细胞的凋亡和抗凋亡之间的不平衡，影响LCSC样细胞的生物学特性。miRNA在LCSC样细胞中起调节作用，其中miR-548 c-5 p可能是抑制因子。
Background. Since the introduction of the theory of tumour stem cells (TSCs), the liver cancer stem cell (LCSC)-like cells have become one of the focuses in the research on liver cancer.Materials and methods. In this study, CD90(+) cells were applied as the possible LCSC-like cells, and the miRNA and gene expression were analyzed in the CD90(+) HepG2 cells. The pilot study showed miR-548c-5p exerted potential effect on the CD90(+) HepG2 cells and was thereafter applied for the further study. CD90(+) HepG2 cells were assigned to miR-548c-5p mimic transfection group and control group. MTT assay was performed to detect the proliferation of CD90(+) HepG2 cells. The migration and invasion abilities were examined by wound healing assay and transwell migration assay, respectively. A detection of apoptosis was performed by fluorescence microscopy.Results. Our results showed that caspase-3 and bcl-2 were down-regulated while caspase-8 was up-regulated in the CD90(+) HepG2 cells. Moreover, the miR-548c-5p transfection could down-regulate the expression of beta-catenin, Tg737, bcl-2, bcl-XL, and caspase-3, inhibit the proliferation, migration and invasion and promote the apoptosis of the CD90(+) HepG2 cells.Conclusions. Our findings indicate the imbalance between apoptosis and anti-apoptosis in the LCSC-like cells, which influence the biological features of LCSC-like cells. miRNA plays a regulatory role in the LCSC-like cells among which miR-548c-5p might be a suppressor.