Effect of Eluent pH on the Ionic and Molecular Forms of the Non-Steroidal Anti-Inflammatory Agents in Reversed-Phase High-Performance Liquid Chromatography
Effect of Eluent pH on the Ionic and Molecular Forms of the Non-Steroidal Anti-Inflammatory Agents in Reversed-Phase High-Performance Liquid Chromatography
复制标题
反相高效液相色谱中洗脱液 pH 对非甾体抗炎药离子和分子形态的影响
DOI：
10.1080/10826079408013484
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发表时间：
1994
影响因子：
1.3
通讯作者：
M. Medenica
中科院分区：
文献类型：
--
作者：
E. Nivaud;M. Guernet;D. Ivanovć;M. Medenica
摘要介绍了几种非甾体抗炎药最佳分离的高效液相色谱条件。研究了被分析化合物的离子(质子化)和分子(非质子化)形式对洗脱液pH的依赖关系。本文研究了几种抗炎药的保留行为与洗脱液pH的关系。对苯酚(扑热息痛、阿司匹林、水杨酰胺、非那西丁和水杨酸)、吡唑烷二酮(磺基吡喃、氧苯丁酮、苯丁酮和酮酮)、氨基-2-烟酸(烟酸和硝普鲁酸)和氨基-2-苯甲酸(甲芬胺和氟苯那酸)的衍生物进行了等梯度反相高效液相色谱分离分析。对含有1%冰醋酸的甲醇-水的流动相进行了不同的变化，以获得最佳分离条件。对于所有被分析物质，计算了在不同的甲醇-水混合溶剂中的pH、pKa和拟合pKa值。
Abstract High-performance liquid chromatographic conditions for the best separation of some non-steroidal anti-inflammatory agents were described. The dependence of eluent pH on the ionic (protonated) and molecular (non-protonated) forms of analysed compounds have been investigated. This paper is the study of the retention behavior of some anti-inflammatory agents depending of the eluent pH. Some derivatives of phenol (acetaminophen, aspirin, salicylamide, phenacetin and salicylic acid), pyrazolidinedione (sulfinpyrazone, oxyphenbutazone, phenylbutazone and ketazone), amino-2-nicotinic acid (nixylic and niflumic acid) and amino-2-benzoic acid (mefenamic and flufenamic acid) were analysed with the isocratic RP-HPLC separation. The different variations of mobile phase methanol-water v/v, containing 1% acetic acid were performed in order to obtain the best conditions of the separation. For all analysed substances the pH, pKa and the fitted pKa values were calculated in different mixtures of methanol-water us...