Role of polymorphisms in the interleukin-10 gene in determining disease susceptibility and phenotype in inflamatory bowel disease
Role of polymorphisms in the interleukin-10 gene in determining disease susceptibility and phenotype in inflamatory bowel disease
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DOI：
10.1023/a:1010604307776
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发表时间：
2001-07-01
影响因子：
3.1
通讯作者：
Hudson, M
中科院分区：
文献类型：
--
作者：
Aithal, GP;Craggs, A;Hudson, M
白细胞介素-10 (IL-10) 在调节粘膜炎症中发挥着关键作用。在 159 名溃疡性结肠炎患者（83 名左侧；76 名广泛性）、90 名克罗恩病患者（22 名小肠；29 名大肠；39 名两者）和 227 名对照者中，评估了 IL-10 基因中 -627 和 -1117 位的功能多态性作为炎症性肠病候选易感位点的作用及其在确定疾病程度方面的重要性。通过 PCR RFLP 测定（-627 位点）或 SSCP 分析（-1117 位点）进行基因分型。与对照组（33%；P = 0.004）相比，在左侧结肠炎患者 (52%) 中观察到过量的 -627A 等位基因，表明 IL-10 可能影响疾病的程度。这些结果并未在新招募的 UC 患者组 (N = 100) 中得到重复。我们得出的结论是，IL-10 基因中 -627 和 -1117 位点的多态性不会影响 IBD 的易感性或决定我们人群中疾病的程度。
Interleukin-10 (IL-10) has a key role in regulating mucosal inflammation. The role of functional polymorphisms at positions -627 and -1117 in the IL-10 gene as candidate susceptibility loci in inflammatory bowel disease and their importance in determining disease extent were evaluated in 159 patients with ulcerative colitis (83 left-sided; 76 extensive), 90 patients with Crohn's disease (22 small bowel; 29 large bowel; 39 both), and 227 controls. Genotyping was performed either by PCR RFLP assays (-627 site) or SSCP analysis (-1117 site). An excess of -627A allele was observed in patients with left-sided colitis (52%) compared with controls (33%; P = 0.004) suggesting that IL-10 may influence the extent of the disease. These results were not replicated in a newly recruited group (N = 100) of patients with UC. We conclude that polymorphisms at -627 and -1117 sites in the IL-10 gene do not contribute to the susceptibility to IBD or determining the extent of the disease in our population.