INTRINSIC VERSUS EXTRINSIC DETERMINANTS DURING THE DEVELOPMENT OF PURKINJE CELL DENDRITES
INTRINSIC VERSUS EXTRINSIC DETERMINANTS DURING THE DEVELOPMENT OF PURKINJE CELL DENDRITES
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DOI：
10.1016/j.neuroscience.2008.12.035
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发表时间：
2009-09-01
期刊：
影响因子：
3.3
通讯作者：
Dusart, I.
中科院分区：
文献类型：
--
作者：
Sotelo, C.;Dusart, I.
浦肯野细胞（PC）树突的特殊形状和分布，平面和高度分枝，为分析神经元树突树获取的细胞和分子调节因子提供了最佳模型。在增殖期结束后的前2周内，细胞的树突经历了2个阶段的重塑过程。第一个阶段包括原始但广泛的树突树的完全缩回，以及从细胞体产生的多个丝状足状突起的形成。在第二阶段，随着成熟树突的快速生长和分支，躯体过程逐渐消失。成熟的浦肯野细胞树突有两种类型的刺状突起，分别称为棘和刺。棘多而长，位于远端树突隔室，与平行纤维形成突触，而刺短而圆，从近端隔室长出，与攀缘纤维形成突触。不同的培养模型和突变小鼠分析表明浦肯野细胞树突发育的内在和外在决定因素的鉴定。树突重构的早期阶段可能是细胞自主的，并受特定转录因子如类维甲酸相关孤儿受体α (ROR α)的调控。传入纤维、营养因子和激素调节成熟树突树的方向和生长，其内在因素尚不清楚，塑造了树突树的总体结构。棘的形成似乎是一种内在现象，独立于它们的突触前伙伴平行纤维，并受沿近端筋室攀爬纤维的抑制影响而局限于远端筋室。(c) 2009 ibro。Elsevier Ltd.出版。版权所有。
The peculiar shape and disposition of Purkinje cell (PC) dendrites, planar and highly branched, offers an optimal model to analyze cellular and molecular regulators for the acquisition of neuronal dendritic trees. During the first 2 weeks after the end of the proliferation period, PCs undergo a 2-phase remodeling process of their dendrites. The first phase consists in the complete retraction of the primitive but extensive dendritic tree, together with the formation of multiple filopodia-like processes arising from the cell body. In the second phase, there is a progressive disappearance of the somatic processes along with rapid growth and branching of the mature dendrite. Mature Purkinje cell dendrites bear two types of spiny protrusions, named spine and thorn. The spines are numerous, elongated, located at the distal dendritic compartment and form synapses with parallel fibers, whereas the thorns are shorter, rounded, emerge from the proximal compartment and synapse with climbing fibers.Different culture models and mutant mice analyses suggest the identification of intrinsic versus extrinsic determinants of the Purkinje cell dendritic development. The early phase of dendritic remodeling might be cell autonomous and regulated by specific transcription factors such as retinoid-related orphan receptor alpha (ROR alpha). Afferent fibers, trophic factors and hormones regulate the orientation and growth of the mature dendritic tree contributing, with still unknown intrinsic factors, to sculpt its general architecture. The formation of spines appears as an intrinsic phenomenon independent of their presynaptic partner, the parallel fibers, and confined to the distal compartment by inhibitory influences of the climbing fibers along the proximal compartment. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.