Internal initiation of translation from the human rhinovirus-2 internal ribosome entry site requires the binding of Unr to two distinct sites on the 5′ untranslated region
Internal initiation of translation from the human rhinovirus-2 internal ribosome entry site requires the binding of Unr to two distinct sites on the 5′ untranslated region
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DOI：
10.1099/vir.0.82463-0
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发表时间：
2007-11-01
影响因子：
3.8
通讯作者：
Jackson, Richard J.
中科院分区：
文献类型：
--
作者：
Anderson, Emma C.;Hunt, Sarah L.;Jackson, Richard J.
人鼻病毒2型（HRV-2）内部核糖体进入位点（IRES）的翻译内部起始依赖于宿主细胞反式作用因子。多重冷休克结构域蛋白Unr和多聚嘧啶，trac-binding蛋白已被鉴定为HRV-2 IRES驱动翻译的协同激活剂。为了研究Unr在这一过程中的作用机制，我们将Unr的结合位点映射到HRV-2 IRES的两个不同的二级结构域，并确定了参与Unr与IRES结合的特定核苷酸。这些数据表明，Unr作为RNA伴侣，以维持翻译能力所需的复杂的三级IRES结构。
Internal initiation of translation from the human rhinovirus-2 (HRV-2) internal ribosome entry site (IRES) is dependent upon host cell trans-acting factors. The multiple cold shock domain protein Unr and the polypyrimidine, tract-binding protein have been identified as synergistic activators of HRV-2 IRES-driven translation. In order to investigate the mechanism by which Unr acts in this process, we have mapped the binding sites of Unr to two distinct secondary structure domains of the HRV-2 IRES, and have identified specific nucleotides that are involved in the binding of Unr to the IRES. The data suggest that Unr acts as an RNA chaperone to maintain a complex tertiary IRES structure required for translational competency.