Cky811 protein expressed by polydnavirus and venom gland of Cotesia kariyai regulates the host Mythimna separata larvae immune response function of C‐type lectin responsible for foreign substance recognition which suppresses its melanization and encapsula
Cky811 protein expressed by polydnavirus and venom gland of Cotesia kariyai regulates the host Mythimna separata larvae immune response function of C‐type lectin responsible for foreign substance recognition which suppresses its melanization and encapsula
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Cky811蛋白由多DNA病毒和Cotesia kariyai毒腺表达，调节宿主粘虫幼虫C型凝集素的免疫应答功能，负责异物识别，抑制其黑化和包膜
DOI：
10.1002/arch.21786
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发表时间：
2021
影响因子：
2.2
通讯作者：
Yutaka Nakamatsu
中科院分区：
文献类型：
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作者：
Tomomi Sawa;Toshiharu Tanaka;Yoshiaki Kato;Yutaka Nakamatsu
植入粘虫幼虫体腔内的Cotesia kariyai (Ck)幼虫在被称为超扩散细胞(HSC)的血细胞粘附后黑色化并被封装。本研究表明，粘虫幼虫注射Ck多DNA病毒（CkPDV） + 毒液（V）后，HSC不能粘附在植入的Ck幼虫上，因此不能发生黑化和包膜。宿主粘虫幼虫的一种称为 Mys-IML 的 C 型凝集素被认为参与了总是在血细胞中表达的外来物质的识别。 CkPDV DNA 编码一种名为 Cky811 的 C 型凝集素，与 Mys-IML 具有高度氨基酸同源性。当 CkPDV + V 在载玻片上与粘虫幼虫的血淋巴混合并在体外培养时，HSC 不会粘附，但添加抗 Cky811 抗体可使 HSC 粘附。注射 CkPDV + V 的粘虫幼虫中 Mys-IML 的信使 RNA (mRNA) 表达在 6 小时内变得不可检测。相反，Cky811 mRNA在注射CkPDV + V 0.5至6小时的粘虫幼虫的血细胞中良好表达。在Ck毒腺 + Ck毒液库的粗提物中也检测到了Cky811蛋白，表明这些蛋白在0.5小时内调节粘虫对异物的识别。这些结果表明，CkPDV + V 抑制 Mys-IML 的 mRNA 表达，而血细胞中表达的 Cky811 蛋白调节 Mys-IML 的外来物质识别，从而抑制下游反应步骤：HSC 粘附、黑化和封装。
Cotesia kariyai(Ck) larvae implanted into the body cavity of theMythimna separata(armyworm) larvae get melanized and encapsulated after adhesion by hemocytes called hyperspread cells (HSCs). The present study showed that HSCs could not adhere to the implanted Ck larvae in armyworm larvae after injection of Ck polydnavirus (CkPDV) + venom (V), thus melanization and encapsulation could not occur. A C‐type lectin called Mys‐IML of the host armyworm larvae was considered to be involved in the recognition of foreign substances which always expressed in hemocytes. The CkPDV DNA encodes a C‐type lectin called Cky811 that has high amino acid homology to Mys‐IML. HSCs did not adhere when CkPDV + V was mixed with the hemolymph of armyworm larvae on glass slides and incubated in vitro, but the addition of anti‐Cky811 antibody enabled HSCs to adhere. The messenger RNA (mRNA) expression of Mys‐IML in armyworm larvae injected with CkPDV + V became undetectable by 6 h. On the contrary, Cky811 mRNA was well expressed in the hemocytes of armyworm larvae injected with CkPDV + V from 0.5 to 6 h. Cky811 protein was also detected in the crude extracts from Ck venom gland + Ck venom reservoir, suggesting that these proteins regulate foreign substance recognition by the armyworm within 0.5 h. These results suggest that CkPDV + V suppresses mRNA expression of Mys‐IML, and that Cky811 protein expressed in hemocytes regulates foreign substance recognition of Mys‐IML, resulting in inhibition of the downstream reaction steps: HSCs adhesion, melanization, and encapsulation.