Effects of ethanol and control liquid diets on the hypothalamic-pituitary-thyroid axis of male Fischer-344 rats.
Effects of ethanol and control liquid diets on the hypothalamic-pituitary-thyroid axis of male Fischer-344 rats.
复制标题
乙醇和对照流质饮食对雄性 Fischer-344 大鼠下丘脑-垂体-甲状腺轴的影响。
DOI：
10.1111/j.1530-0277.1992.tb00709.x
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
PrangeJr,AJ
中科院分区：
文献类型：
--
作者：
Mason,GA;Noonan,LR;Garbutt,JC;Caldwell,JD;Shimoda,K;Walker,CH;Li,L;PrangeJr,AJ
在成年雄性Fischer-344（F-344）大鼠组中研究了含乙醇的高葡萄糖流质饮食和两种不同对照流质饮食对血清和脑甲状腺轴激素以及肝和脑脱碘酶活性的影响。大鼠自由采食实验室食物;自由采食营养完全的10%（w/v）乙醇液体饲料;与给予乙醇饲料的大鼠消耗的饲料体积相等体积的高碳水化合物（HC）或高脂肪（HF）等热量对照液体饲料;或自由采食HC对照饲料。在整个研究期间，每天测量流质饲料的消耗量，每隔一天记录一次体重。在2、4或8周后测量激素，并在4或8周后测量脱碘酶活性。此外，各组大鼠被给予10%乙醇饮食，随意自由进食，或成对间断喂食HC对照饮食8周，测定甲状腺激素和促甲状腺激素（TSH）对促甲状腺激素释放激素（TRH）的反应。在2周内，大鼠习惯了所有饮食，此后各组的体重增加相当。饲喂乙醇饲料的大鼠与配对饲喂HC或HF对照大鼠的血清甲状腺激素之间的微小差异可能是由饲喂乙醇的大鼠的T4分泌降低引起的。喂食流质饲料4或8周的F-344大鼠与喂食实验室饲料的大鼠之间游离和总T4和T3的显著差异可能是由于喂食流质饲料的大鼠肝脏5′-脱碘酶活性较高所致。Sprague-道利大鼠的甲状腺激素水平与F-344大鼠相似，但饮食诱导的变化不太明显。在喂食乙醇液体饲料的F-344大鼠、配对喂食HC对照饲料的大鼠或接受实验室饲料的大鼠中，未观察到T4或T3的大脑皮质水平存在差异。在间歇饲喂乙醇饲料、对照饲料或实验室饲料的F-344大鼠中，未观察到基础甲状腺轴激素或TSH对TRH的反应存在显著差异。
The effects of a high dextrose liquid diet containing ethanol and two different control liquid diets on serum and brain thyroid axis hormones and liver and brain deiodinase activities were studied in groups of adult male Fischer‐344 (F‐344) rats. Rats received either lab chow, ad libitum; a nutritionally complete 10% (w/v) ethanol liquid diet, ad libitum; a volume of either a high carbohydrate (HC) or a high fat (HF) isocaloric control liquid diet equal to the volume of diet consumed by rats given the ethanol diet; or the HC control diet, ad libitum. Consumption of liquid diets was measured daily and body weights recorded every other day throughout the study. Hormones were measured after 2, 4, or 8 weeks and deiodinase activities after 4 or 8 weeks. Also, groups of rats were given the 10% ethanol diet, ad libitum, or pair‐fed the HC control diet intermittently for 8 weeks, and thyroid hormones and thyroid‐stimulating hormone (TSH) response to thyrotropin‐releasing hormone (TRH) were determined.Within 2 weeks rats became accustomed to all diets and thereafter weight gain was comparable in all groups. Small differences between serum thyroid hormones of rats fed the ethanol diet and pair‐fed HC or HF controls may have been caused by lower T4secretion in ethanol‐fed rats. Marked differences in free and total T4and T3between F‐344 rats fed liquid diets for 4 or 8 weeks and rats fed lab chow probably resulted from higher liver 5′‐deiodinase activity in rats fed liquid diets. Sprague‐Dawley rats showed similar but less marked dietary‐induced changes in thyroid hormone levels than did F‐344 rats. No differences in cerebral cortical levels of T4or T3were observed among F‐344 rats fed the ethanol liquid diet, rats pair‐fed HC control diet, or rats that received lab chow. No significant differences in basal thyroid axis hormones or TSH response to TRH were observed among F‐344 rats intermittently fed ethanol diet, control diet, or lab chow.