Gradual Loss of a DNA-Inducible Protein Kinase Activity From the Cytoplasmic Extracts of Arbacia Embryos.
Gradual Loss of a DNA-Inducible Protein Kinase Activity From the Cytoplasmic Extracts of Arbacia Embryos.
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Arbacia 胚胎细胞质提取物中 DNA 诱导的蛋白激酶活性逐渐丧失。
DOI：
10.1086/bblv191n2p281
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Rasmussen,Howard
中科院分区：
文献类型：
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作者：
Kanungo,Jyotshnabala;Calhoun,Benjamin;Takeda,Yoshihiko;Hardin,JohnA;Rasmussen,Howard
DNA依赖性蛋白激酶是丝氨酸/苏氨酸蛋白激酶，其活性需要有切口的双链（ds）DNA（I）。它在DNA修复中发挥着至关重要的作用（2），但其在发育中的作用尚不清楚。据报道，在培养的人类细胞和几种海洋无脊椎动物的卵母细胞或胚胎的提取物中发现了DNA活化蛋白磷酸化（3）。在此，我们表明，海胆（Arbacia punctdata）卵的细胞质提取物中含有一种激酶，该激酶仅在受精后才能由外源性双链DNA诱导，并且在囊胚，原肠胚和plutei制备的细胞质提取物中检测不到。
The DNA-dependent protein kinase is a serine/threonine protein kinase that requires nicked double-stranded (ds) DNA for its activity (I). It plays a crucial role in DNA repair (2) but its role in development is not clear. DNA-activated protein phosphorylation has been reported in extracts from cultured human cells and the oocytes or the embryos of several marine invertebrates (3). Herein, we show that the cytoplasmic extracts of sea urchin (Arbacia punctdata) eggs contain a kinase that is inducible by exogenous dsDNA only after fertilization and is not detectable in cytoplasmic extracts prepared from blastulae, gastrulae, and plutei.