Phenylpropanoids from maize pericarp: resistance factors to kernel infection and fumonisin accumulation by Fusarium verticillioides
Phenylpropanoids from maize pericarp: resistance factors to kernel infection and fumonisin accumulation by Fusarium verticillioides
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DOI：
10.1007/s10658-012-0069-3
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发表时间：
2012
影响因子：
1.8
通讯作者：
D. Sampietro;C. M. Fauguel;M. Vattuone;D. Presello;C. Catalán
中科院分区：
文献类型：
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作者：
D. Sampietro;C. M. Fauguel;M. Vattuone;D. Presello;C. Catalán
这项工作的目的是研究果皮苯丙素作为 F 抗性因子的作用。十一种玉米基因型中的轮枝菌。接种F后测量疾病严重程度和籽粒伏马菌素积累量。在两年进行的田间试验中，轮枝菌和与果皮苯丙素含量有关。谷物伏马菌素浓度高度依赖于基因型的疾病严重程度（r= 0.88）。对果皮苯丙素的详细分析表明存在反式阿魏酸 (tFA)、顺式阿魏酸 (cFA)、对香豆酸 (pCA) 和五种二阿魏酸 (DFA)。最突出的二阿魏酸盐是 8,5'-二阿魏酸苯并呋喃形式 (8,5'-DFAbz)，其次是 8,5'-DFA 和 8,8'-DFA。除cFA外，最具抗性的基因型表现出高水平的苯丙素，这与疾病严重程度和谷物伏马菌素浓度的低水平有关（-0.61>r> -0.90）。逐步回归分析显示，总差异是疾病严重程度变异性的最佳解释参数（r2= 0.71）。总二阿魏酸、8,5'DFAbz 和 pCA 的含量很好地描述了谷物伏马菌素浓度 (r2=≥0.82)。我们的研究结果表明，仁果皮中高水平的苯丙素是与较少的疾病严重程度和由F.引起的伏马菌素积累相关的性状。轮枝菌。进一步的研究正在进行中，以绘制本研究中包含的抗性和易感基因型交叉衍生的双亲群体中这些细胞壁成分的数量性状位点。
The aim of this work was to investigate the role of pericarp phenylpropanoids as resistance factors toF. verticillioidesin eleven maize genotypes. Disease severity and kernel fumonisin accumulation were measured after inoculation withF. verticillioidesand related to contents of pericarp phenylpropanoids in field trials conducted during 2 years. Grain fumonisin concentrations were highly dependant on disease severity of the genotypes (r= 0.88). A detailed analysis of pericarp phenylpropanoids indicated the presence oftrans-ferulic acid (tFA),cis-ferulic acid (cFA), p-coumaric acid (pCA), and five diferulates (DFAs). The most prominent diferulates were 8,5′-diferulic acid benzofuram form (8,5′-DFAbz), followed by 8,5′-DFA and 8,8′-DFA. Except for cFA, the most resistant genotypes exhibited high levels of phenylpropanoids which were related to low levels of disease severity and grain fumonisin concentration (−0.61 >r> −0.90). A stepwise regression analysis revealed that total diferulates was the best explanatory parameter for variability of disease severity (r2= 0.71). Grain fumonisin concentration was well depicted by contents of total diferulates, 8,5′DFAbz and pCA (r2= 0.82). Our findings suggest that high level of phenylpropanoids in the kernel pericarp is a trait associated to less disease severity and fumonisin accumulation caused byF. verticillioides. Further research is in progress to map quantitative trait loci for these cell wall components in bi-parental populations derived by crossing resistant and susceptible genotypes included in this study.