Molecular beacons: Probes that fluoresce upon hybridization
Molecular beacons: Probes that fluoresce upon hybridization
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DOI：
10.1038/nbt0396-303
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发表时间：
1996-03-01
影响因子：
46.9
通讯作者：
Kramer, FR
中科院分区：
文献类型：
--
作者：
Tyagi, S;Kramer, FR
我们已经开发了新型的核酸探针，其识别并报告均质溶液中特定核酸的存在。当这些探针与其靶杂交时，它们经历自发的荧光构象变化。只有完全互补的靶标才会引发这种反应，因为当靶标含有错配的核苷酸或缺失时，不会发生杂交。所述探针特别适合于真实的实时监测特定核酸的合成。当用于核酸扩增测定时，基因检测是均质的和灵敏的，并且可以在密封管中进行。当引入活细胞时，这些探针应该能够追踪特定mRNA的起源，运动和命运。
We have developed novel nucleic acid probes that recognize and report the presence of specific nucleic acids in homogeneous solutions. These probes undergo a spontaneous fluorogenic conformational change when they hybridize to their targets. Only perfectly complementary targets elicit this response, as hybridization does not occur when the target contains a mismatched nucleotide or a deletion. The probes are particularly suited for monitoring the synthesis of specific nucleic acids in real time. When used in nucleic acid amplification assays, gene detection is homogeneous and sensitive, and can be carried out in a sealed tube. When introduced into living cells, these probes should enable the origin, movement, and fate of specific mRNAs to be traced.