DIFFERENTIATION OF ADULT LEYDIG-CELLS
DIFFERENTIATION OF ADULT LEYDIG-CELLS
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DOI：
10.1016/0960-0760(95)00022-r
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发表时间：
1995-06-01
影响因子：
4.1
通讯作者：
HARDY, MP
中科院分区：
文献类型：
--
作者：
BENTON, L;SHAN, LX;HARDY, MP
成年间质细胞出生后起源于睾丸内。它们的形成是一个连续的过程，涉及祖细胞逐渐转化为成熟细胞类型。尽管这些变化的渐进性质，在大鼠间质细胞的增殖，分化和类固醇功能的研究，导致承认三个不同的发育阶段，在成年间质细胞系：间质细胞祖细胞，未成熟的间质细胞和成年间质细胞。在第一阶段，Leydig细胞祖细胞产生于出生后第三周期间睾丸间质中间充质样干细胞的活跃增殖，并且可以通过Leydig细胞标记物的存在来识别，例如3 β-羟基类固醇脱氢酶（3 β-HSD）的组织化学染色和黄体生成素（LH）受体的存在。它们活跃地增殖，并在产后第28天分化成未成熟的Leydig细胞。在第二阶段，未成熟的Leydig细胞在形态上可识别为Leydig细胞。它们具有丰富的滑面内质网并且具有类固醇生成活性，但主要产生5 α还原型雄激素而不是睾酮。未成熟的Leydig细胞只分裂一次，产生总的成年Leydig细胞群。在第三和最后阶段，成年Leydig细胞完全分化，主要产生睾酮，很少分裂。LH和雄激素共同作用，刺激Leydig细胞祖细胞分化为未成熟Leydig细胞。初步的数据表明，胰岛素样生长因子-1（IGF-1）的行为随后在未成熟的Leydig细胞转化成成年Leydig细胞。
Adult Leydig cells originate within the testis postnatally. Their formation is a continuous process involving gradual transformation of progenitors into the mature cell type. Despite the gradual nature of these changes, studies of proliferation, differentiation and steroidogenic function in the rat Leydig cell led to the recognition of three distinct developmental stages in the adult Leydig cell lineage: Leydig cell progenitors, immature Leydig cells and adult Leydig cells. In the first stage, Leydig cell progenitors arise from active proliferation of mesenchymal-like stem cells in the testicular interstitium during the third week of postnatal life and are recognizable by the presence of Leydig cell markers such as histochemical staining for 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) and the present of luteinizing hormone (LH) receptors. They proliferate actively and by day 28 postpartum differentiate into immature Leydig cells. In the second stage, immature Leydig cells are morphologically recognizable as Leydig cells. They have an abundant smooth endoplasmic reticulum and are steroidogenically active, but primarily produce 5 alpha-reduced androgens rather than testosterone. Immature Leydig cells divide only once, giving rise to the total adult Leydig cell population. In the third and final stage, adult Leydig cells are fully differentiated, primarily produce testosterone and rarely divide. LH and androgen act together to stimulate differentiation of Leydig cell progenitors into immature Leydig cells. Preliminary data indicate that insulin like growth factor-1 (IGF-1) acts subsequently in the transformation of immature Leydig cells into adult Leydig cells.