COMPETENCE AND DETERMINATION IN THE PROCESS OF INVITRO SHOOT ORGANOGENESIS
COMPETENCE AND DETERMINATION IN THE PROCESS OF INVITRO SHOOT ORGANOGENESIS
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DOI：
10.1016/0012-1606(83)90029-5
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发表时间：
1983-01-01
影响因子：
2.7
通讯作者：
WARNICK, DA
中科院分区：
文献类型：
--
作者：
CHRISTIANSON, ML;WARNICK, DA
当用 Murashige 和 Skoog 盐、蔗糖、维生素和 0.05 mg/l IAA 加 7.0 mg/l 2-异戊烯基腺嘌呤培养时，空心菜的叶外植体会产生芽。芽、根或愈伤组织诱导培养基（SIM、RIM 或 CIM）将导致在外植体切割边缘形成少量愈伤组织。这种第一次形成的愈伤组织在发育上是可互换的：SIM 在 CIM 或 SIM 上形成的愈伤组织中诱导芽，具有相同的效果和效率。一旦在有能力的愈伤组织中开始诱导，愈伤组织就不再可以互换。在 SIM 的持续影响下，细胞或细胞群决定芽的形成。这一决定对于芽的形成有很强的影响：随后转移到生根培养基不会影响外植体芽的形成。生长素/细胞分裂素平衡对器官发生的控制必须发生在组织变得有能力的时间和确定芽（或根）发育的时间之间。目前尚不清楚这种控制是单一现象还是多重现象。
Leaf explants of Convolvulus arvensis produce shoots when cultured on Murashige and Skoog salts, sucrose, vitamins and 0.05 mg/l IAA plus 7.0 mg/l 2-isopentenyl adenine. Shoot-, root- or callus-inducing medium (SIM, RIM or CIM) will cause small amounts of callus to form at the cut edges of the explant. This 1st-formed callus is developmentally interchangeable: SIM induces shoots in callus formed on CIM or SIM with equal effect and efficiency. Once induction begins in competent callus, the callus is no longer interchangeable. Under the continued influence of SIM, cells or groups of cells become determined for shoot formation. This determination is strongly canalized for shoot formation: subsequent transfer to root-inducing medium does not affect the formation of shoots by the explant. The control of organogenesis by the auxin/cytokinin balance must occur between the time the tissue becomes competent and the time it is determined for shoot (or root) development. It is not known whether this control is a single or multiple phenomenon.