RANDOM AMPLIFIED POLYMORPHIC DNA-FINGERPRINTING OF CAMPYLOBACTER-JEJUNI AND CAMPYLOBACTER-COLI ISOLATED FROM HUMAN FECES, SEAWATER AND POULTRY PRODUCTS
RANDOM AMPLIFIED POLYMORPHIC DNA-FINGERPRINTING OF CAMPYLOBACTER-JEJUNI AND CAMPYLOBACTER-COLI ISOLATED FROM HUMAN FECES, SEAWATER AND POULTRY PRODUCTS
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DOI：
10.1016/0923-2508(96)81065-5
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发表时间：
1995-10-01
影响因子：
2.6
通讯作者：
OWEN, RJ
中科院分区：
文献类型：
--
作者：
HERNANDEZ, J;FAYOS, A;OWEN, RJ
应用聚合酶链式反应技术对食品、海水和人粪便中分离的空肠弯曲菌和大肠埃希氏菌的型和血清型参考菌株进行了随机扩增多态性DNA分析。对供试菌株进行了遗传多样性分析，共获得118条不同的随机扩增带，每个谱带的大小在0.30~1.50kb之间，每个谱带的条带数为4~11条。对随机10聚体引物(序列5‘-CAATCGCCGT-3’)的鉴别力进行了评估。一般来说，相同Penner血清组的菌株没有共同的谱带，但来自不同Penner血清型的偶尔菌株共享相同的带型。经数值分析后，在40%的相似性水平上确定了5个主要的类群，分别对应于空肠梭菌、卷曲梭菌和幼虫，但有一些例外。弯曲杆菌的随机扩增多态性DNA图谱具有很高的区分性，是流行病学研究中传统分型的一种有价值的新选择。
The polymerase chain reaction (PCR) technique was used to obtain randomly amplified polymorphic DNA (RAPD) profiles from 64 type and serotype reference strains and 114 isolates of Campylobacter jejuni and C. coli from food, seawater and human faeces. Genetic diversity was detected among the strains asa total of 118 different RAPD profiles were obtained, each one containing from 4 to 11 bands between 0.30 and 1.50 kb. The discriminatory power of a random 10-mer primer (sequence 5'-CAATCGCCGT-3') was assessed. In general, no profiles were common to strains of the same Penner serogroup, but occasional strains from different Penner serotypes shared identical band profiles. RAPD analysis also differentiated between the species, and after numerical analysis, five main clusters were defined at the 40% similarity level, corresponding to C. jejuni, C. coil and C. lari with some exceptions. RAPD profiling of Campylobacter is highly discriminatory and is a valuable new alternative to traditional typing in epidemiological studies.