Ability of an avirulent mutant of Vibrio cholerae to colonize in the infant mouse upper bowel.
Ability of an avirulent mutant of Vibrio cholerae to colonize in the infant mouse upper bowel.
复制标题
霍乱弧菌无毒突变体在幼年小鼠上肠中定殖的能力。
DOI：
10.1128/iai.32.2.474-479.1981
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发表时间：
1981
影响因子：
3.1
通讯作者：
Parker,CD
中科院分区：
文献类型：
--
作者：
Sigel,SP;Finkelstein,RA;Parker,CD
霍乱弧菌菌株3083（生物型El Tor，血清型Ogawa）和得克萨斯星-SR（SR），一种衍生自3083的突变体，产生霍乱原的B（结合）但不产生A（毒性）亚基，比较了它们的能力：（i）与幼鼠上肠结合;（ii）在那里存活和繁殖;和（iii）诱导腹泻。用35 SO 4标记的弧菌用于确定与上肠的关联和繁殖能力。亲本菌株的相关性显着优于SR，虽然活的突变体细胞被发现在幼鼠肠16至18小时后的挑战。外源毒素的加入提高了标记SR（但不是3083）被清除的速率，进一步表明SR与上肠的关联性较差。SR和3083都在上肠繁殖，但可能是由于前3小时内轻微的净杀伤及其更快的清除率，SR在攻毒后8小时的种群规模仅为3083的10%。菌株3083在幼鼠中引起腹泻，但SR没有，即使在通过幼鼠肠道连续传代10次后。菌株SR在37和40 ℃下对温度稍敏感。它作为活疫苗的潜在用途进行了讨论。
Vibrio cholerae strain 3083 (biotype El Tor, serotype Ogawa) and Texas Star-SR (SR), a mutant derived from 3083 that produces the B (binding) but not the A (toxic) subunit of choleragen, were compared in their abilities to: (i) associate with the infant mouse upper bowel; (ii) survive and multiple there; and (iii) induce diarrhea. Vibrios labeled with 35SO4 were used to determine association with the upper bowel and ability to multiply. The parental strain associated significantly better than SR, although viable mutant cells were found in the infant mouse intestine 16 to 18 h after challenge. Addition of exogenous toxin enhanced the rate at which labeled SR (but not 3083) was cleared, further suggesting that SR associates less well with the upper bowel. Both SR and 3083 multiplied in the upper bowel but, due perhaps to slight net killing during the first 3 h and its more rapid rate of clearance, SR achieved a population size only 10% that of 3083 by 8 h postchallenge. Strain 3083 elicited diarrhea in infant mice but SR did not, even after 10 successive passages through the infant mouse intestine. Strain SR was slightly temperature sensitive at 37 and 40 degrees C. Its potential use as a live vaccine is discussed.