Biomimetic Remineralization of Carious Lesions by Self-Assembling Peptide
Biomimetic Remineralization of Carious Lesions by Self-Assembling Peptide
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DOI：
10.1177/0022034517698419
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发表时间：
2017-07-01
影响因子：
7.6
通讯作者：
Pieles, U.
中科院分区：
文献类型：
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作者：
Kind, L.;Stevanovic, S.;Pieles, U.
龋齿是世界上最常见的疾病。为预防和确定龋齿的再生治疗方案，已经作出了巨大的努力。自组装的薄片形成肽先前已被证明可以形成支持组织再生的三维纤维网络。特别是，自组装肽P-11-4在治疗和预防龋齿方面显示出潜力。以前的研究表明，应用单体P-11-4溶液治疗早期龋齿病变可以通过形成新的羟基磷灰石晶体来增加净矿物增益。这种作用模式的假设是单体自组装肽P-11-4扩散到表面下病变体中，并在其中组装成高级原纤维，通过模仿牙釉质的自然生物矿化促进表面下体积的矿化，并作为新形成的羟基磷灰石内置的支架留在病变体中。本研究旨在探讨自组装肽P-11-4支持牙釉质矿化的作用机制。通过各种分析方法，可以表明自组装肽P-11-4扩散到表面下病变，在整个病变体积中组装成更高形状的聚集体，并支持新生羟基磷灰石纳米晶体的成核，从而导致表面下龋齿病变内的矿物质密度增加。结果表明，自组装肽P-11-4的应用可以通过支持牙釉质自然形成的矿化来促进牙釉质病变的表面下再生，其作用模式与牙釉质的自然形成相似。
Caries is the most common disease in the world. Great efforts have been undertaken for prevention and to identify a regenerative treatment solution for dental caries. Self-assembling -sheet forming peptides have previously shown to form 3-dimensional fiber networks supporting tissue regeneration. In particular, the self-assembling peptide P-11-4 has shown potential in the treatment and prevention of dental caries. It has previously been shown that application of monomeric P-11-4 solution to early carious lesions can increase net mineral gain by forming de novo hydroxyapatite crystals. The hypothesis for the mode of action was that monomeric self-assembling peptide P-11-4 diffuses into the subsurface lesion body and assembles therein into higher order fibrils, facilitating mineralization of the subsurface volume by mimicking the natural biomineralization of the tooth enamel, and it remains within the lesion body as a scaffold built-in by the newly formed hydroxyapatite. The aim of the present study was to investigate the mechanism of action of the self-assembling peptide P-11-4 supporting mineralization of carious enamel. By various analytical methods, it could be shown that the self-assembling peptide P-11-4 diffuses into the subsurface lesion, assembles into higher formed aggregates throughout the whole volume of the lesion, and supports nucleation of de novo hydroxyapatite nanocrystals and consequently results in increased mineral density within the subsurface carious lesion. The results showed that the application of self-assembling peptide P-11-4 can facilitate the subsurface regeneration of the enamel lesion by supporting de novo mineralization in a similar mode of action as has been shown for the natural formation of dental enamel.