Highly efficient enzymatic synthesis of 3'deoxyapionucleic acid(apioNA) having the four natural nucleobases

Highly efficient enzymatic synthesis of 3'deoxyapionucleic acid(apioNA) having the four natural nucleobases
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高效酶法合成具有四种天然核碱基的 3 脱氧阿普核酸(apioNA)

DOI:
10.1039/c1cc12980e
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发表时间:
2011
期刊:
Chem.Commun.
影响因子:
--
通讯作者:
M.
M.
中科院分区:
--
文献类型:
--
作者:
Kataoka;M.

文献摘要

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The synthesis of the 3′-deoxyapionucleoside 3′′-triphosphates (apioNTPs) having the four natural nucleobases and their enzymatic incorporation into a DNA–DNA primer–template have been tried. Therminator DNA polymerase was shown to incorporate these apioNTPs effectively giving 43mer DNA–apioNA chimera.