Collection and fixation of spider embryos.
Collection and fixation of spider embryos.
复制标题
蜘蛛胚胎的收集和固定。
DOI：
10.1101/pdb.prot5067
复制
发表时间：
2008
期刊：
影响因子：
--
通讯作者：
W. Damen
中科院分区：
文献类型：
--
作者：
Nikola;M. Schoppmeier;W. Damen
简介蜘蛛Cupiennius salei，通常被称为美国流浪蜘蛛，是一个特别有用的胚胎学研究的实验室模型，因为可用的工具来研究和操纵其胚胎发育。Cupiennius用于研究轴的形成，分割，附属物的发展，神经发生和丝绸生产。这些研究有助于我们理解这些过程的进化，但它们也有助于我们理解进化新奇事物的起源和多样性。蜘蛛和昆虫的比较可以显示节肢动物进化过程中发育机制的保守程度和分歧。蜘蛛和昆虫之间保存的任何胚胎学特征都可能代表节肢动物的祖先特征。昆虫和蜘蛛的比较分子胚胎学工作最终应该使我们能够为节肢动物门定义一个分子原型。这本身将是比较不同后生动物门，包括脊索动物的必要基石。本方案描述了从C. salei。固定的胚胎可以在-20 ° C下保存较长时间，并用于原位杂交，用于使用末端脱氧核苷酸转移酶介导的dUTP-地高辛缺口末端标记（TUNEL）的细胞凋亡研究，以及用于免疫组织化学。
INTRODUCTIONThe spider Cupiennius salei, commonly known as the American wandering spider, is a particularly useful laboratory model for embryological studies because of the availability of tools to study and manipulate its embryonic development. Cupiennius is used to study axis formation, segmentation, appendage development, neurogenesis, and silk production. These studies contribute to our understanding of the evolution of these processes, but they also help us to understand the origin and diversification of evolutionary novelties. Comparisons between spiders and insects can show the degree of conservation and divergence of developmental mechanisms during arthropod evolution. Any embryological feature conserved between spiders and insects is likely to represent an ancestral feature for arthropods. Comparative molecular embryological work in insects and spiders should eventually allow us to define a molecular archetype for the phylum Arthropoda. This in itself will be a necessary cornerstone for comparing the different metazoan phyla, including chordates. This protocol describes the collection and fixation of embryos from C. salei. The fixed embryos can be stored at -20°C for prolonged periods and used for in situ hybridization, in studies of apoptosis using terminal deoxynucleotidyl-transferase-mediated dUTP-digoxigenin nick-end labeling (TUNEL), and for immunohistochemistry.