Collagen containing neonatal astrocytes stimulates regrowth of injured fibers and promotes modest locomotor recovery after spinal cord injury
Collagen containing neonatal astrocytes stimulates regrowth of injured fibers and promotes modest locomotor recovery after spinal cord injury
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DOI：
10.1002/jnr.20088
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发表时间：
2004-07-01
影响因子：
4.2
通讯作者：
Hamers, FPT
中科院分区：
文献类型：
--
作者：
Joosten, EAJ;Veldhuis, WB;Hamers, FPT
使用胶原作为ET载体将新生星形胶质细胞移植到成年大鼠损伤的脊髓中，可以精确地将这些细胞应用到损伤间隙中，并最大限度地减少移植细胞的迁移。这种方法可能会导致解剖和功能的恢复。在本研究中，20只成年雌性Wistar大鼠接受了胸段脊髓水平的背侧半横断。将培养的新生大鼠皮质星形胶质细胞以胶原为载体移植到损伤处(N=10)。移植前，用坚牢蓝标记培养的星形胶质细胞。对照组大鼠仅接受胶原植入(N=10)。存活1个月后，连续监测大鼠功能恢复情况。组织学数据显示，预先标记的星形胶质细胞在移植后存活，并主要定位于胶原植入物中。几乎没有快速蓝色标记的GFAP阳性星形胶质细胞从植入物迁移到邻近的宿主脊髓。移植的新生星形胶质细胞的存在导致植入植入物的神经丝阳性纤维(包括顺行标记的皮质脊髓轴突)的数量显著增加。植入纤维与移植的星形胶质细胞密切相关。植入新生星形胶质细胞确实导致了在开阔场地运动分析(BBB评分)或穿越人行道(T型台)时观察到的运动恢复的轻微暂时改善。(C)2004年Wiley-Liss公司
The use of collagen as et vehicle to transplant neonatal astroglial cells into the lesioned spinal cord of the adult rat allows a precise application of these cells into the lesion gap and minimizes the migration of the transplanted cells. This approach might lead to anatomical and functional recovery. In the present study, 20 adult female Wistar rats were subjected to a dorsal hemisection at thoracic spinal cord levels. Cultured cortical neonatal rat astrocytes were transplanted into the lesion with collagen as a vehicle (N = 10). Prior to transplantation, the cultured astroglial cells were labelled with fast blue. Control rats received collagen implants only (N = 10). During 1 month of survival time, functional recovery of all rats was continuously monitored. Histological data showed that the prelabelled astroglial cells survived transplantation and were localized predominantly in the collagen implant. Virtually no fast blue-labelled GFAP-positive astroglial cells migrated out of the implant into the adjacent host spinal cord. The presence of transplanted neonatal astroglial cells resulted in a significant increase in the number of ingrowing neurofilament-positive fibers (including anterogradely labeled corticospinal axons) into the implant. Ingrowing fibers were closely associated with the transplanted astroglial cells. The implantation of neonatal astroglial cells did result in modest temporary improvements of locomotor recovery as observed during open-field locomotion analysis (BBB subscore) or during crossing of a walkway (catwalk). (C) 2004 Wiley-Liss, Inc.