Assessment of mutations induced by cold atmospheric plasma jet treatment relative to known mutagens in Escherichia coli.
Assessment of mutations induced by cold atmospheric plasma jet treatment relative to known mutagens in Escherichia coli.
复制标题
相对于大肠杆菌中已知的诱变剂，评估冷大气等离子体射流处理诱导的突变。
DOI：
10.1093/mutage/geab030
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发表时间：
2021
期刊：
影响因子：
2.7
通讯作者：
Patenall BL
中科院分区：
文献类型：
--
作者：
Patenall BL
冷大气等离子体（CAP）的主要杀菌成分被认为是活性氧和氮类（RONS）和紫外线辐射，这两者都有能力引起DNA损伤和突变。本文以X射线和紫外线照射为对照，研究了CAP对大肠杆菌的诱变作用。DNA损伤和诱变分别使用基于扩散的DNA片段化试验和改良的艾姆斯试验进行筛选。对从后者获得的突变菌落进行定量和测序。发现CAP引起与X-照射相似的突变谱，这与UV不相似，这意味着CAP产生的RONS更可能是CAP的致突变组分。CAP治疗也显示出促进对抗生素环丙沙星的耐药性。我们的数据表明，CAP治疗有致突变作用，可能有重要的表型后果。
The main bactericidal components of cold atmospheric plasma (CAP) are thought to be reactive oxygen and nitrogen species (RONS) and UV-radiation, both of which have the capacity to cause DNA damage and mutations. Here, the mutagenic effects of CAP onEscherichia coliwere assessed in comparison to X- and UV-irradiation. DNA damage and mutagenesis were screened for using a diffusion-based DNA fragmentation assay and modified Ames test, respectively. Mutant colonies obtained from the latter were quantitated and sequenced. CAP was found to elicit a similar mutation spectrum to X-irradiation, which did not resemble that for UV implying that CAP-produced RONS are more likely the mutagenic component of CAP. CAP treatment was also shown to promote resistance to the antibiotic ciprofloxacin. Our data suggest that CAP treatment has mutagenic effects that may have important phenotypic consequences.