Theoretical model and experimental study of red blood cell (RBC) deformation in microchannels
Theoretical model and experimental study of red blood cell (RBC) deformation in microchannels
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DOI：
10.1016/j.jbiomech.2006.10.004
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发表时间：
2007-01-01
影响因子：
2.4
通讯作者：
Dinnar, Uri
中科院分区：
文献类型：
--
作者：
Korin, Natanel;Bransky, Avishay;Dinnar, Uri
利用理论模型和一种新型的自动流变仪研究了红细胞在微通道中的运动和变形。建立了预测胞体在剪切作用下变形随胞体几何形状和力学性能变化的理论模型。结合流体力学和膜力学，以迭代的方式计算牵引力和变形。利用该模型评估了不同生物物理参数（如细胞内粘度、膜剪切模量和表面体积比）对变形测量的影响。该实验系统能够测量微通道内特定平面上单个红细胞的速度及其变形。在模拟结果、流变仪测量结果和已发表的ektacyometry结果之间观察到良好的一致性。理论模型结果表明，这种可变形性测量技术受电池内部粘度或周围流体粘度变化的影响很小。然而，这些测量对RBC剪切模量非常敏感。由模型和流变仪测量得到的剪切模量介于微移管吸吸和激光捕获得到的值之间。该研究展示了理论模型与微制造装置的集成，以便更好地理解RBC力学及其使用微流体剪切试验的测量。该系统和模型有潜力作为定量诊断红细胞变形性障碍的临床工具。(C) 2006 Elsevier Ltd版权所有。
The motion and deformation of red blood cells (RBCs) flowing in a microchannel were studied using a theoretical model and a novel automated rheoscope. The theoretical model was developed to predict the cells deformation under shear as a function of the cells geometry and mechanical properties. Fluid dynamics and membrane mechanics are incorporated, calculating the traction and deformation in an iterative manner. The model was utilized to evaluate the effect of different biophysical parameters, such as: inner cell viscosity, membrane shear modulus and surface to volume ratio on deformation measurements.The experimental system enables the measurement of individual RBCs velocity and their deformation at defined planes within the microchannel. Good agreement was observed between the simulation results, the rheoscope measurements and published ektacytometry results. The theoretical model results imply that such deformability measuring techniques are weakly influenced by changes in the inner viscosity of the cell or the ambient fluid viscosity. However, these measurements are highly sensitive to RBC shear modulus. The shear modulus, estimated by the model and the rheoscope measurements, falls between the values obtained by micropipette aspiration and laser trapping.The study demonstrates the integration of a theoretical model with a microfabricated device in order to achieve a better understanding of RBC mechanics and their measurement using microfluidic shear assays. The system and the model have the potential of serving as quantitative clinical tools for diagnosing deformability disorders in RBCs. (C) 2006 Elsevier Ltd. All rights reserved.